1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. SUC2 蛋白, Saccharomyces cerevisiae (P.pastoris, His)

SUC2 蛋白, Saccharomyces cerevisiae (P.pastoris, His)

目录号: HY-P74534
产品使用指南

SUC2 基因是酿酒酵母基因组的一个非连锁基因座。SUC2 蛋白, Saccharomyces cerevisiae (P.pastoris, His) 是重组的 SUC2 蛋白,由 P. pastoris 表达,带有 C-His 标签。SUC2 蛋白, Saccharomyces cerevisiae (P.pastoris, His) 全长 513 个氨基酸,分子量约为 ~60 kDa。

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描述

SUC2 gene is a unlinked loci of the S. cerevisiae genome[1]. SUC2 Protein, Saccharomyces cerevisiae (P.pastoris, His) is the recombinant SUC2 protein, expressed by P. pastoris , with C-His labeled tag. The total length of SUC2 Protein, Saccharomyces cerevisiae (P.pastoris, His) is 513 a.a., with molecular weight of ~60 kDa.

研究背景

SUC2 基因是酿酒酵母基因组的一个非连锁基因座[1]。SUC2 基因编码两种形式的转化酶:一种是分泌型糖基化形式,其合成受到葡萄糖抑制的调节,另一种是组成型产生的细胞内非糖基化酶[2]。SUC2 蛋白, Saccharomyces cerevisiae (P.pastoris, His) 具有 β- 呋喃果糖苷酶活性和菊粉酶活性,并参与菊粉、棉子糖和蔗糖的分解代谢[3][4][5]

种属

Others

表达系统

P. pastoris

标签

C-His

蛋白编号

P00724 (S20-K532)

基因 ID

854644  [NCBI]

蛋白结构
N-term
SUC2 (S20-K532)
Accession # P00724
His
C-term
同用名
Invertase 2; Saccharase; SUC2; Beta-fructofuranosidase 2
分子量

Approximately 60 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SUC2 Protein, Saccharomyces cerevisiae (P.pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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