SULT1A1 蛋白, Human (His)

SULT1A1 Protein, Human (His) is the recombinant human-derived SULT1A1 protein, expressed by E. coli, with N-6*His labeled tag.

SULT1A1 是一种磺基转移酶，利用 3'-phospho-5'-adenylyl sulfate (PAPS) 作为磺酸供体，催化多种带有羟基或胺基的受体分子的硫酸化结合反应。硫酸化可增加大多数化合物的水溶性，从而促进其肾脏排泄，但也可能导致生物活化形成活性代谢物。该酶对小分子酚类化合物表现出广泛的底物特异性，在类固醇激素等内源性分子的硫酸化中起重要作用（如 dopamine、甲状腺激素 T4、T3、rT3 和 3,3'-T2，其中对 3,3'-T2 的催化活性最高）。此外，SULT1A1 介导外源性物质（如药物 acetaminophen 和 minoxidil，by similar）的硫酸化，并参与致癌性 N-hydroxyarylamines 的代谢活化，形成可导致 DNA 加合物的高活性中间体。最新研究表明，该酶可能通过 O-硫酸化肠道菌群代谢产物 4-ethylphenol (4-EP) 影响血脑屏障通透性和少突胶质细胞成熟，进而调节大脑边缘系统的功能连接。

Measured by its ability to transfer sulfate from PAPS to 1-Napthol. The specific activity is 56.4 pmol/min/μg.

Human

E. coli

N-6*His

AAH00923.1 (M1-L295)

6817  [NCBI]

Full Length

MELIQDTSRPPLEYVKGVPLIKYFAEALGPLQSFQARPDDLLISTYPKSGTTWVSQILDMIYQGGDLEKCHRAPIFMRVPFLEFKAPGIPSGMETLKDTPAPRLLKTHLPLALLPQTLLDQKVKVVYVARNAKDVAVSYYHFYHMAKVHPEPGTWDSFLEKFMVGEVSYGSWYQHVQEWWELSRTHPVLYLFYEDMKENPKREIQKILEFVGHSLPEETVDFVVQHTSFKEMKKNPMTNYTTVPQEFMDHSISPFMRKGMAGDWKTTFTVAQNERFDADYAEKMAGCSLSFRSEL

Approximately 32-34 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM PB, 10% Trehalose, 50 mM Nacl, 0.05% Tween 80, pH 7.8 or 50 mM PB, 50 mM NaCl, pH 7.8, 10% Glycerol, 10% trehalose and 0.05% Tween80.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.