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SUMO2 蛋白, Human (His)

目录号: HY-P70974
COA 产品使用指南

小泛素相关修饰因子 2 (SUMO2) 是一种泛素样蛋白,作为翻译后修饰系统的一部分共价附着在蛋白质上。SUMO2 参与多种细胞过程,如核转运、转录调控、凋亡、DNA 复制和修复、有丝分裂、信号转导和蛋白质稳定性等。SUMO2 蛋白, Human (His) 是重组的 SUMO2 蛋白,由 E. coli 表达,带有 N-6*His 标签。SUMO2 蛋白, Human (His) 全长 93 个氨基酸,分子量约为 ~17.0 kDa。

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规格 价格 是否有货 数量
10 μg ¥350 In-stock
50 μg ¥980 In-stock
100 μg   询价  

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描述

Small ubiquitin-related modifier 2 (SUMO2) is a ubiquitin-like protein that covalently attached to proteins as part of post-translational modification system. SUMO2 is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, DNA replication and repair, mitosis, signal transduction and protein stability. SUMO2 Protein, Human (His) is the recombinant human-derived SUMO2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of SUMO2 Protein, Human (His) is 93 a.a., with molecular weight of ~17.0 kDa.

研究背景

小泛素相关修饰因子 2 (SUMO2) 是 SUMO 蛋白家族的成员。SUMO2 是一种泛素样蛋白,可以作为单体或赖氨酸连接的聚合物共价附着在蛋白质上,是翻译后修饰系统的一部分。SUMO2 参与多种细胞过程,如核转运、转录调控、凋亡、DNA 复制和修复、有丝分裂、信号转导和蛋白质稳定性等。直到羧基末端的最后两个氨基酸被切割掉,SUMO2 才有活性。聚合 SUMO2 链也易受多泛素化的影响,多泛素化是蛋白酶体降解被修饰蛋白的信号。SUMO2 也在调节 SETX 的苏酰化状态中发挥作用。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

AAH08450.1 (M1-G93)

基因 ID
蛋白结构
N-term
6*His
SUMO2 (M1-G93)
Accession # AAH08450.1
C-term
同用名
Small Ubiquitin-Related Modifier 2; SUMO-2; HSMT3; SMT3 homolog 2; SUMO-3; Sentrin-2; Ubiquitin-Like Protein SMT3A; Smt3A; SUMO2; SMT3A; SMT3H2
氨基酸序列

MADEKPKEGVKTENNNHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG

分子量

Approximately 17.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

SUMO2 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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