SUV420H2 蛋白, Human (His)

SUV420H2 protein is a histone methyltransferase that methylates H4K20 to generate H4K20me3 and H4K20me2, which are critical for transcriptional regulation and genome integrity. SUV420H2 functions in pericentric heterochromatin, establishes constitutive heterochromatin, and interacts with RB1 family proteins. SUV420H2 Protein, Human (His) is the recombinant human-derived SUV420H2 protein, expressed by E. coli , with N-His labeled tag. The total length of SUV420H2 Protein, Human (His) is 279 a.a., with molecular weight of ~60 kDa.

SUV420H2 蛋白是一种组蛋白甲基转移酶，在甲基化组蛋白 H4 的单甲基化“Lys-20”(H4K20me1) 和二甲基化“Lys-20”(H4K20me2) 方面表现出特异性，产生三甲基化“Lys-20”(H4K20me3) 和二甲基化“Lys-20” ' (H4K20me2)，从而有助于转录调节和基因组完整性的维持。在体外，它还展示了甲基化组蛋白 H4 和核小体的未修饰“Lys-20”(H4K20me0) 的能力。H4“Lys-20”三甲基化充当与转录抑制相关的特定表观遗传标签。SUV420H2 主要在中心周异染色质区域发挥作用，在这些基因组区域建立组成型异染色质方面发挥着核心作用。该酶通过与 RB1 家族蛋白（RB1、RBL1 和 RBL2）相互作用而靶向组蛋白 H3，在 DNA 损伤时促进 TP53BP1 焦点形成，通过二甲基化和三甲基化促进熟练的非同源末端连接 (NHEJ) 定向 DNA 修复组蛋白 H4 的“Lys-20”。此外，它可能通过催化组蛋白 H4 的“Lys-20”的二甲基化和三甲基化（通过相似性）来促进类别转换重组。

Human

E. coli

N-His

Q86Y97 (G2-L280)

84787  [NCBI]

GPDRVTARELCENDDLATSLVLDPYLGFRTHKMNVSPVPPLRRQQHLRSALETFLRQRDLEAAYRALTLGGWTARYFQSRGPRQEAALKTHVYRYLRAFLPESGFTILPCTRYSMETNGAKIVSTRAWKKNEKLELLVGCIAELREADEGLLRAGENDFSIMYSTRKRSAQLWLGPAAFINHDCKPNCKFVPADGNAACVKVLRDIEPGDEVTCFYGEGFFGEKNEHCECHTCERKGEGAFRTRPREPALPPRPLDKYQLRETKRRLQQGLDSGSRQGL

Approximately 60 kDa

Greater than 80% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 500 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.