T-PA 蛋白, Human (HEK293)

The T-PA protein encoded by this gene is a secreted serine protease that converts plasminogen into plasmin, a key enzyme involved in tissue remodeling and cell migration. The proteolytic process produces heavy and light chains, forming heterodimeric enzymes. T-PA Protein, Human (HEK293) is the recombinant human-derived T-PA protein, expressed by HEK293 , with tag free. The total length of T-PA Protein, Human (HEK293) is 252 a.a., with molecular weight of ~35 kDa.

T-PA 蛋白由该基因编码，是一种分泌型丝氨酸蛋白酶，对于纤溶酶原转化为纤溶酶至关重要，纤溶酶是参与组织重塑和细胞迁移的关键纤溶酶。前蛋白原经过纤溶酶或胰蛋白酶的蛋白水解加工，产生通过二硫键连接形成异二聚酶的重链和轻链。T-PA 的酶活性在维持纤维蛋白溶解和凝血之间的微妙平衡方面发挥着关键作用。活动增加会导致纤溶亢进，导致出血过多，而活动减少会导致纤溶低下，可能导致血栓形成或栓塞。选择性剪接产生多种转录变体，包括至少一种经过蛋白水解加工的同种型。该基因表现出广泛的表达，在膀胱 (RPKM 61.1)、胎盘 (RPKM 41.3) 和其他 18 个组织中观察到显着水平，反映了其在各种生理环境中的不同作用。

Measured by its ability to cleave a peptide substrate, Z-Gly-Gly-Arg-AMC. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read). The specific activity is 3403.37 pmol/min/µg, as measured under the described conditions.

Human

HEK293

Tag Free

NP_000921.1 (I311-P562)

5327  [NCBI]

IKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWIRDNMRP

Approximately 35 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 10 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.