T4 DNA Polymerase 蛋白, T4 phage (His)

T4 DNA Polymerase, a pivotal enzyme in viral DNA replication, performs dual functions crucial for genome synthesis. As a polymerase, it synthesizes DNA, and with its exonucleolytic activity, it proofreads by degrading single-stranded DNA in the 3'- to 5'-direction, ensuring the accuracy and fidelity of replicated viral genomic DNA. T4 DNA Polymerase Protein, T4 phage (His) is the recombinant T4 DNA Polymerase protein, expressed by E. coli , with N-6*His labeled tag. T4 DNA Polymerase Protein, T4 phage (His), has molecular weight of ~103.6 kDa.

T4 DNA 聚合酶是病毒 DNA 复制中的关键酶，具有对其在基因组合成中的作用至关重要的双重酶功能。该聚合酶展示了 DNA 合成（充当聚合酶）和核酸外切活性，促进单链 DNA 在 3' 至 5' 方向上的降解。后一个功能用于校对目的，确保复制的病毒基因组 DNA 的准确性和保真度。

The specific activity is ≥3000 U/mL.Unit Definition:One unit is defined as the amount of enzyme that catalyzes the incorporation of deoxyribonucleotids(dNTPs) into polynucleotides at 37 ºC for 30 minutes. Reaction Buffer: 50 mM NaCl, 10 mM Tris-HCl, 10 mM MgCl, PH 7.9 at 25℃.

Others

E. coli

N-6*His

P04415

1258685  [NCBI]

MKEFYISIETVGNNIVERYIDENGKERTREVEYLPTMFRHCKEESKYKDIYGKNCAPQKFPSMKDARDWMKRMEDIGLEALGMNDFKLAYISDTYGSEIVYDRKFVRVANCDIEVTGDKFPDPMKAEYEIDAITHYDSIDDRFYVFDLLNSMYGSVSKWDAKLAAKLDCEGGDEVPQEILDRVIYMPFDNERDMLMEYINLWEQKRPAIFTGWNIEGFDVPYIMNRVKMILGERSMKRFSPIGRVKSKLIQNMYGSKEIYSIDGVSILDYLDLYKKFAFTNLPSFSLESVAQHETKKGKLPYDGPINKLRETNHQRYISYNIIDVESVQAIDKIRGFIDLVLSMSYYAKMPFSGVMSPIKTWDAIIFNSLKGEHKVIPQQGSHVKQSFPGAFVFEPKPIARRYIMSFDLTSLYPSIIRQVNISPETIRGQFKVHPIHEYIAGTAPKPSDEYSCSPNGWMYDKHQEGIIPKEIAKVFFQRKDWKKKMFAEEMNAEAIKKIIMKGAGSCSTKPEVERYVKFSDDFLNELSNYTESVLNSLIEECEKAATLANTNQLNRKILINSLYGALGNIHFRYYDLRNATAITIFGQVGIQWIARKINEYLNKVCGTNDEDFIAAGDTDSVYVCVDKVIEKVGLDRFKEQNDLVEFMNQFGKKKMEPMIDVAYRELCDYMNNREHLMHMDREAISCPPLGSKGVGGFWKAKKRYALNVYDMEDKRFAEPHLKIMGMETQQSSTPKAVQEALEESIRRILQEGEESVQEYYKNFEKEYRQLDYKVIAEVKTANDIAKYDDKGWPGFKCPFHIRGVLTYRRAVSGLGVAPILDGNKVMVLPLREGNPFGDKCIAWPSGTELPKEIRSDVLSWIDHSTLFQKSFVKPLAGMCESAGMDYEEKASLDFLFG

Approximately 103.6 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 100 mM KPO4 , 1 mM DTT , 50 % Glycerol, (pH 6.5 @ 25°C)

N/A.

Stored at -20°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -20°C for extended storage. Avoid repeated freeze-thaw cycles.

Room temperature in continental US; may vary elsewhere.