TFEB 蛋白, Human (N-His)

TFEB Protein, a transcription factor, masterfully regulates lysosomal functions, autophagy, lipid catabolism, energy metabolism, and immune responses. Recognizing E-box and CLEAR-box sequences, it controls gene expression in response to nutrient availability. MTOR phosphorylation inhibits TFEB, retaining it cytosolic, while starvation induces dephosphorylation, leading to nuclear localization and gene activation. TFEB promotes autophagy and plays a role in immune responses, including antibacterial defense. The drug eltrombopag inhibits TFEB by binding to its bHLH domain, disrupting DNA-binding. TFEB protein, Human (N-His) is the recombinant human-derived TFEB protein, expressed by E. coli , with N-His labeled tag. The total length of TFEB protein, Human (N-His) is 476 a.a., with molecular weight of ~57.0 kDa.

TFEB 蛋白作为关键转录因子协调多种细胞过程，包括溶酶体生物发生、自噬、溶酶体胞吐作用、脂质分解代谢、能量代谢和免疫反应。识别 E-box 序列后，TFEB 的有效 DNA 结合需要与其自身或其他 MiT/TFE 家族成员（如 TFE3 或 MITF）进行二聚化。它通过 MTOR 介导的磷酸化对氨基酸可用性做出反应，促进细胞质保留和失活。相反，饥饿或溶酶体应激期间的 MTOR 抑制会诱导 TFEB 去磷酸化，从而导致核定位和转录激活。 TFEB 特异性结合 CLEAR-box 序列，激活溶酶体基因的表达并调节溶酶体定位。此外，它还能积极调节自噬，促进 T 细胞依赖性抗体反应，并在胎盘血管化中发挥作用。为了应对细菌感染，TFEB 介导衣康酸诱导的烷基化，激活溶酶体生物发生并帮助细菌清除。值得注意的是，艾曲波帕药物通过与 bHLH 结构域结合并破坏 DNA 结合来抑制 TFEB。

Human

E. coli

N-His

P19484-1(M1-L476)

7942

MASRIGLRMQLMREQAQQEEQRERMQQQAVMHYMQQQQQQQQQQLGGPPTPAINTPVHFQSPPPVPGEVLKVQSYLENPTSYHLQQSQHQKVREYLSETYGNKFAAHISPAQGSPKPPPAASPGVRAGHVLSSSAGNSAPNSPMAMLHIGSNPERELDDVIDNIMRLDDVLGYINPEMQMPNTLPLSSSHLNVYSSDPQVTASLVGVTSSSCPADLTQKRELTDAESRALAKERQKKDNHNLIERRRRFNINDRIKELGMLIPKANDLDVRWNKGTILKASVDYIRRMQKDLQKSRELENHSRRLEMTNKQLWLRIQELEMQARVHGLPTTSPSGMNMAELAQQVVKQELPSEEGPGEALMLGAEVPDPEPLPALPPQAPLPLPTQPPSPFHHLDFSHSLSFGGREDEGPPGYPEPLAPGHGSPFPSLSKKDLDLMLLDDSLLPLASDPLLSTMSPEASKASSRRSSFSMEEGDVL

Approximately 57.0 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from sterile 20 mM PB, 150 mM Nacl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.