TFPI2 蛋白, Human (HEK293, C-His)

The critical role of the TFPI2 protein in regulating plasmin-mediated matrix remodeling suggests its involvement in extracellular matrix dynamics. It inhibits trypsin, plasmin, factor VIIa/tissue factor and weak factor Xa, affecting coagulation and fibrinolysis. TFPI2 Protein, Human (HEK293, C-His) is the recombinant human-derived TFPI2 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of TFPI2 Protein, Human (HEK293, C-His) is 191 a.a., with molecular weight of approximately 43 kDa.

TFPI2 蛋白可能在纤溶酶介导的基质重塑的调节中发挥至关重要的作用，这表明它参与了控制细胞外基质动力学的复杂过程。TFPI2 对胰蛋白酶、纤溶酶、VIIa 因子/组织因子具有抑制作用，对 Xa 因子具有较弱的抑制作用，表明其对凝血和纤溶中涉及的各种蛋白水解活性具有潜在影响。值得注意的是，TFPI2 不影响凝血酶。它与 ABCB1、TFPI2 和 PPP2R3C 形成复合物，导致 ABCB1 去磷酸化。这种复杂的形成暗示了涉及 TFPI2 调节 ABCB1 磷酸化状态的潜在调节机制，这可能对细胞过程产生更广泛的影响。进一步探索 TFPI2 抑制功能的具体机制和下游效应及其在含有 ABCB1 的复合物中的相互作用，可以为了解其在止血和基质重塑调节中的多方面作用提供有价值的见解。

Measured by its ability to inhibit proliferation of A549 human lung carcinoma cells. The ED₅₀ this effect is 43.15 ng/mL, corresponding to a specific activity is 2.32×10⁴ units/mg.

Human

HEK293

C-10*His

P48307 (D23-K213)

7980  [NCBI]

DAAQEPTGNNAEICLLPLDYGPCRALLLRYYYDRYTQSCRQFLYGGCEGNANNFYTWEACDDACWRIEKVPKVCRLQVSVDDQCEGSTEKYFFNLSSMTCEKFFSGGCHRNRIENRFPDEATCMGFCAPKKIPSFCYSPKDEGLCSANVTRYYFNPRYRTCDAFTYTGCGGNDNNFVSREDCKRACAKALK

Approximately 18-32 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.