2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. TGS1 蛋白, Human (His-SUMO)

TGS1 蛋白, Human (His-SUMO)

目录号: HY-P71608
产品使用指南

TGS1 蛋白催化小核 RNA (snRNA) 和小核仁 RNA (snoRNA) 的 7-单甲基鸟苷 (m(7)G) 帽转化为 2, 2,7-三甲基鸟苷 (m( 2,2,7)G ) 帽结构。该酶对鸟嘌呤表现出特异性，在修饰过程中，N7 甲基化发生在 N2 甲基化之前。TGS1 蛋白, Human (His-SUMO) 是重组的 TGS1 蛋白，由 E. coli 表达，带有 N-His, N-SUMO 标签。TGS1 蛋白, Human (His-SUMO) 全长 141 个氨基酸，分子量约为 ~31.6 kDa。

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描述

TGS1 protein catalyzes the conversion of the 7-monomethylguanosine (m(7)G) cap of small nuclear RNA (snRNA) and small nucleolar RNA (snoRNA) into 2, 2,7-trimethylguanosine (m( 2,2,7)G) Cap structure. The enzyme shows specificity for guanine, with N7 methylation occurring before N2 methylation during the modification process. TGS1 Protein, Human (His-SUMO) is the recombinant human-derived TGS1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of TGS1 Protein, Human (His-SUMO) is 141 a.a., with molecular weight of ~31.6 kDa.
研究背景

TGS1 或三甲基鸟苷合酶 1 是一种关键酶，可催化两个连续的甲基化步骤，这些步骤对于将小核 RNA (snRNA) 和小核仁 RNA (snoRNA) 上存在的 7-单甲基鸟苷 (m7G) 帽转化为 2,2、 7-三甲基鸟苷 (m2,2,7G) 帽结构。该过程涉及鸟嘌呤在 N7 位点的特异性甲基化，然后是 N2 甲基化。U snRNA 上 m7G 帽的高甲基化导致它们集中在核灶中，与线圈蛋白共定位，并形成典型的卡哈尔小体 (CB)。TGS1 参与这些过程表明它在转录调节和核结构动态组织中的作用。它必须强调 TGS1 对鸟嘌呤的特异性及其在塑造 RNA 分子帽结构、影响与 RNA 修饰和定位相关的细胞过程中的关键功能。
种属

Human
表达系统

E. coli
标签

N-His;N-SUMO
蛋白编号

Q96RS0 (713M-853T)
基因 ID
蛋白结构
N-term
6*His-SUMO
TGS1 (713M-853T)
Accession # Q96RS0
C-term
同用名
Cap specific guanine N2 methyltransferase; Cap-specific guanine-N2 methyltransferase; CLL associated antigen KW 2; CLL-associated antigen KW-2; DKFZp762A163; FLJ22995; HCA137; Hepatocellular carcinoma associated antigen 137; Hepatocellular carcinoma-associated antigen 137; NCOA6IP; Nuclear receptor coactivator 6 interacting protein; Nuclear receptor coactivator 6-interacting protein; PIMT; PIPMT; PRIP interacting protein PIPMT; PRIP interacting protein with methyltransferase domain; PRIP interacting protein with methyltransferase motif; PRIP-interacting protein with methyltransferase motif; SEREX defined; TGS 1; Tgs1; TGS1_HUMAN; Trimethylguanosine synthase; Trimethylguanosine synthase homolog (S. cerevisiae); Trimethylguanosine synthase homolog
氨基酸序列

MRVIAIDIDPVKIALARNNAEVYGIADKIEFICGDFLLLASFLKADVVFLSPPWGGPDYATAETFDIRTMMSPDGFEIFRLSKKITNNIVYFLPRNADIDQVASLAGPGGQVEIEQNFLNNKLKTITAYFGDLIRRPASET

分子量

Approximately 31.6 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

TGS1 Protein, Human (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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