Thioredoxin/TRX 蛋白, Mouse (N-His)

Thioredoxin/TRX Protein engages in diverse redox reactions, utilizing its active dithiol center for reversible oxidation and disulfide formation. It catalyzes essential dithiol-disulfide exchanges and plays a crucial role in S-nitrosylation of cysteine residues, responding to intracellular nitric oxide. Thioredoxin inhibits CASP3 activity by nitrosylating its active site cysteine. It also regulates FOS/JUN AP-1 DNA binding activity and influences interleukin-2 receptor expression, showcasing its multifaceted cellular role beyond redox regulation. Thioredoxin/TRX Protein, Mouse (N-His) is the recombinant mouse-derived Thioredoxin/TRX protein, expressed by E. coli, with N-6*His labeled tag. The total length of Thioredoxin/TRX Protein, Mouse (N-His) is 105 a.a., with molecular weight of ~15 kDa.

硫氧还蛋白/TRX蛋白积极参与多种氧化还原反应，利用其活性中心二硫醇进行可逆氧化和二硫键形成，从而催化重要的二硫醇-二硫键交换反应。除了其经典的氧化还原功能外，硫氧还蛋白在靶蛋白内半胱氨酸残基的可逆 S-亚硝基化中发挥着至关重要的作用，有助于细胞对细胞内一氧化氮的反应。值得注意的是，它响应一氧化氮而亚硝基化 CASP3 的活性位点半胱氨酸，有效抑制 caspase-3 活性。此外，硫氧还蛋白对电离辐射细胞中的 FOS/JUN AP-1 DNA 结合活性具有调节影响，通过其氧化还原状态调节 AP-1 转录活性。此外，硫氧还蛋白还参与白细胞介素 2 受体 TAC (IL2R/P55) 表达的增强，强调了其在氧化还原调节之外的细胞过程中的多方面作用。

Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation, which can be measured by absorbance at 650 nm. The specific activity is 5.603 A650/min/mg, as measured under the described conditions.

Mouse

E. coli

N-6*His

P10639 (M1-A105)

22166  [NCBI]

MVKLIESKEAFQEALAAAGDKLVVVDFSATWCGPCKMIKPFFHSLCDKYSNVVFLEVDVDDCQDVAADCEVKCMPTFQFYKKGQKVGEFSGANKEKLEASITEYA

Approximately 15 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.