1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens Receptor Proteins Enzymes & Regulators
  3. Angiopoietins Endothelial cell CD Proteins Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. TIE-2/CD202b TIE Receptors
  5. TIE-2
  6. TIE-2 蛋白, Cynomolgus (HEK293, Fc)

TIE-2 蛋白, Cynomolgus (HEK293, Fc)

目录号: HY-P76673
COA 产品使用指南

TIE-2 属于 RTPK 家族蛋白,是 I 型跨膜蛋白. TIE-2 可以作为生长因子、分化因子和刺激代谢反应因子的受体。TIE-2 蛋白, Cynomolgus (HEK293, Fc) 是重组的 TIE-2 蛋白,由 HEK293 表达,带有 C-hFc 标签。TIE-2 蛋白, Cynomolgus (HEK293, Fc) 全长 723 个氨基酸,分子量约为 ~130 kDa。

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规格 价格 是否有货 数量
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2 μg ¥350 In-stock
10 μg ¥720 In-stock
50 μg ¥2000 In-stock
100 μg ¥3400 In-stock
500 μg ¥10200 In-stock
1 mg ¥17300 In-stock
> 100 μg   询价  

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描述

TIE-2 belongs to the RTPK family of proteins and is a type I transmembrane protein. TIE-2 can serve as a receptor for growth factors, differentiation factors, and metabolic response stimulators. TIE-2 Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived TIE-2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TIE-2 Protein, Cynomolgus (HEK293, Fc) is 723 a.a., with molecular weight of ~130 kDa.

研究背景

TIE-2 属于 RTPK 家族蛋白,是 I 型跨膜蛋白. TIE-2 可以作为生长因子、分化因子和刺激代谢反应因子的受体[1]

生物活性

Immobilized Cynomolgus TIE-2 at 1 μg/mL (100 μL/well) can bind Biotinylated Angiopoietin-2. The ED50 for this effect is 88.60 ng/mL.

  • Immobilized Cynomolgus TIE-2 at 1 μg/mL (100 μL/well) can bind Biotinylated Angiopoietin-2. The ED50 for this effect is 88.60 ng/mL.
种属

Cynomolgus

表达系统

HEK293

标签

C-hFc

蛋白编号

G7PS68 (A23-K745)

基因 ID

/

蛋白结构
N-term
TIE-2 (A23-K745)
Accession # G7PS68
hFc
C-term
同用名
Angiopoietin-1 receptor; CD202b antigen; CD202b; hTIE2; p140 TEK; TEK; Tie-2; VMCM; VMCM1
氨基酸序列

AMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGEAIRIRTMKMRQQASFLPATLTMTVDKGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPECNRLCTVCVNNGVCHEDTGECICPPGFMGRTCEKACERHTFGRTCKERCSGQDGCKSYVFCLPDPYGCSCATGWKGLQCNEACHHGFYGPDCKLRCSCSNGETCDRFQGCLCSPGRQGLQCEREGIPRMTPKIVDLPDHIEVNSGKFNPICKASGWPLPTNEEMTLVKPDGTVLHPKDFNHTDHFSVAIFTIHRILPPDSGVWVCSANTVAGMVEKPFNISVKVLPKPLNAPNVIDTGHNFAVINISSEPYFGDGPIKSKKLLYKPVNHYEAWRHIQVTNEIVTLNHLEPRTEYELCVQLVRRGEGGEGHPGPVRRFTTASIGLPPPRGLNLLPKSQTTLNLTWQPIFPSSEDDFYVEVERRSVQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARVNTKAQGEWSEDLTAWTLSDILPPQPENIKISNITHSSAVISWTILDGYSISSITIRYKVQGKNEDQHIDVKIKNATITQYQLKGLEPETAYQVDIFAENNIGSSNPAFSHELVTLPESEAPADLGGGK

分子量

Approximately 130 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TIE-2 Protein, Cynomolgus (HEK293, Fc)
目录号:
HY-P76673
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