TIE-2 蛋白, Mouse (HEK293, His)

TIE-2 Protein, Mouse (HEK293, His) belongs to the receptor tyrosine kinase family and functions as a receptor for Angiopoietin-1 (Ang1). Ang1 bridges TIE-2 at cell-cell contacts, resulting in trans-association of TIE-2 in the presence of cell-cell contacts. TIE-2 activation promotes vessel assembly and maturation by mediating survival signals for EC and regulating the recruitment of pericytes. TIE-2 maintains the vascular integrity of mature vessels by enhancing endothelial barrier function and inhibiting apoptosis of endothelial cells^[1][2][3]. TIE-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived TIE-2 protein, expressed by HEK293 , with C-His labeled tag. The total length of TIE-2 Protein, Mouse (HEK293, His) is 722 a.a., with molecular weight of 95-120 kDa.

TIE-2 由 TEK 基因编码，是一种酪氨酸激酶受体，在血管稳定性中发挥核心作用。Ang1/Tie2 信号传导被认为可以调节血管静止的维持和血管生成的促进^[1]。
血管生成素 (Ang)/Tie2 系统是血管生物学的关键调节因子。膜结合 (mb) Tie2 和 Ang-1 的表达可确保血管稳定性，而 Ang-2 可通过血管内皮生长因子 (VEGF)、缺氧和炎症诱导，充当拮抗剂。Tie2 信号传导也会被可溶性 Tie2 (sTie2) 减弱，sTie2 是受体的胞外结构域，在 VEGF 刺激后会脱落^[2]。

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Immobilized Mouse Angiopoietin-2 at 4 μg/mL (100 μL/well) can bind Biotinylated TIE2. The ED₅₀ for this effect is 460.2 ng/mL.

• 
  Immobilized Mouse Angiopoietin-2 at 4 μg/mL (100 μL/well) can bind Biotinylated TIE2. The ED₅₀ for this effect is 460.2 ng/mL.

Mouse

HEK293

C-His

B1AWS8 (A23-K744)

21687  [NCBI]

AMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGQAIRIRTMKMRQQASFLPATLTMTVDRGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPDCSRPCTTCKNNGVCHEDTGECICPPGFMGRTCEKACEPHTFGRTCKERCSGPEGCKSYVFCLPDPYGCSCATGWRGLQCNEACPSGYYGPDCKLRCHCTNEEICDRFQGCLCSQGWQGLQCEKEGRPRMTPQIEDLPDHIEVNSGKFNPICKASGWPLPTSEEMTLVKPDGTVLQPNDFNYTDRFSVAIFTVNRVLPPDSGVWVCSVNTVAGMVEKPFNISVKVLPEPLHAPNVIDTGHNFAIINISSEPYFGDGPIKSKKLFYKPVNQAWKYIEVTNEIFTLNYLEPRTDYELCVQLARPGEGGEGHPGPVRRFTTASIGLPPPRGLSLLPKSQTALNLTWQPIFTNSEDEFYVEVERRSLQTTSDQQNIKVPGNLTSVLLSNLVPREQYTVRARVNTKAQGEWSEELRAWTLSDILPPQPENIKISNITDSTAMVSWTIVDGYSISSIIIRYKVQGKNEDQHIDVKIKNATVTQYQLKGLEPETTYHVDIFAENNIGSSNPAFSHELRTLPHSPASADLGGGK

95-120 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.