1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens Receptor Proteins Enzymes & Regulators
  3. Angiopoietins Endothelial cell CD Proteins Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. TIE-2/CD202b TIE Receptors
  5. TIE-2
  6. TIE-2 蛋白, Rat (HEK293, Fc)

TIE-2 蛋白, Rat (HEK293, Fc)

目录号: HY-P73438
COA 产品使用指南

TIE-2 蛋白具有生长因子结合活性,并参与骨化、cAMP 反应和雌激素反应。预计它存在于多种细胞成分中,作为质膜的成分和受体复合物的一部分。TIE-2 蛋白, Rat (HEK293, Fc) 是重组的 TIE-2 蛋白,由 HEK293 表达,带有 C-hFc 标签。TIE-2 蛋白, Rat (HEK293, Fc) 全长 721 个氨基酸,分子量为 120-130 kDa。

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规格 价格 是否有货 数量
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2 μg ¥300 In-stock
10 μg ¥580 In-stock
50 μg ¥1460 In-stock
100 μg ¥2200 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

TIE-2 protein has growth factor binding activity and is involved in ossification, cAMP response, and estrogen response. It is predicted to be present in a variety of cellular components, as a component of the plasma membrane, and as part of receptor complexes. TIE-2 Protein, Rat (HEK293, Fc) is the recombinant rat-derived TIE-2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TIE-2 Protein, Rat (HEK293, Fc) is 721 a.a., with molecular weight of 120-130 kDa.

研究背景

TIE-2 蛋白具有生长因子结合活性,并参与多种过程,包括软骨内骨化、cAMP 反应和雌激素反应。预计它位于不同的细胞成分中,例如基底外侧质膜、中心粒卫星和微绒毛,并且预计是质膜的组成部分和受体复合物的一部分。预测的与肌动蛋白丝和应力纤维的共定位表明它可能参与细胞骨架动力学。值得注意的是,TIE-2 可作为多种疾病的生物标志物,包括急性肾小管坏死、脑缺血、胃溃疡、甲状腺肿和肝细胞癌。该基因的人类直系同源物与动静脉畸形和多发性皮肤和粘膜静脉畸形有关。TIE-2 的表达存在偏差,在肺 (RPKM 772.5)、心脏 (RPKM 237.7) 和各种其他组织中观察到显着水平。

生物活性

Immobilized Rat Tie-2 at 1 μg/mL (100 μL/well) can bind Biotinylated Angiopoietin-2. The ED50 for this effect is 22.89 ng/mL.

种属

Rat

表达系统

HEK293

标签

C-hFc

蛋白编号

NP_001099207.1 (A23-L743)

基因 ID

89804  [NCBI]

蛋白结构
N-term
TIE-2 (A23-L743)
Accession # NP_001099207.1
hFc
C-term
同用名
Angiopoietin-1 receptor; CD202b; hTIE2; p140 TEK; Tie2; VMCM
氨基酸序列

AMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGQAIRIRTMKMRQQASFLPATLTMTVDRGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPDCNRPCTTCKNNGVCHEDTGECICPPGFMGRTCEKACEPHTFGRTCKERCSGSEGCKSYVFCLPDPYGCSCATGWRGLQCNEACPYGHYGPDCKLRCHCTNEEMCDRFQGCLCSQGWQGLQCEKEGRPRMTPQIEDLPDHIEVNSGKFNPICKASGWPLPTSEEMTLVKPDGTVLQPNDFNHTDHFSVAIFTVNRILPPDSGVWVCSVNTVAGMVEKPFNISVKVLPEPLHAPNVIDTGHNFAIINISSEPYFGDGPIKSKKLFYKPVNQAWKYIQVMNEIVTLNYLEPRTDYELCVQLVRPGEGGEGHPGPVRRFTTASIGLPPPRGLSLLPKSQTALNLTWQPIFTSSEDEFYVEVERWSQQTRSDQQNIKVPGNLTSVLLNNLLPREQYSVRARVNTKAQGEWSEELRAWTLSDILPPQPENIKITNITDYTALVSWTIVDGYSISSIIIRYKVQGKNEDQHIDVKIKNATITQYQLKGLEPETTYHVDIFAENNIGSSNPAFSQEIRTLPAPKDLGGGKML

分子量

Approximately 115-130 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TIE-2 Protein, Rat (HEK293, Fc)
目录号:
HY-P73438
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