TIM-1/KIM-1/HAVCR 蛋白, Canine (HEK293, His)

TIM-1/KIM-1/HAVCR Protein, Canine (HEK293, His) is the recombinant canine-derived TIM-1/KIM-1/HAVCR, expressed by HEK293 , with N-His labeled tag. ,

TIM-1 属于含有类免疫球蛋白结构域的跨膜蛋白家族，包括人类中的 3 个成员 (人 TIM1、TIM3 和 TIM4) 和小鼠中的 8 个成员 (鼠 TIM1 至 TIM8)，其中人 TIM1、TIM3 和 TIM4 分别是鼠 TIM1、TIM3 和 TIM4 的直接同源物。这些蛋白质在细胞表面表达，其 N 端免疫球蛋白样 (IgV) 和粘蛋白结构域存在于细胞外环境中，其 C 端序列存在于细胞质中。所有 TIM 蛋白的一个重要特征是 IgV 结构域中高度保守的磷脂酰丝氨酸 (PtdSer) 结合袋，可识别凋亡细胞外膜小叶上的 PtdSer，促进吞噬细胞对其的摄取^[1]。
TIM-1 是一种 I 型膜蛋白，具有 IgV 结构域，后跟重度糖基化的粘蛋白结构域、跨膜结构域和带有一个酪氨酸磷酸化基序的细胞内胞质尾部。TIM-1可以作为T细胞激活的共刺激分子。TIM-1 与抗体交联，结合 TCR 和 CD28 刺激，可增强 CD4+ T 细胞的增殖。TIM-1 的过度表达会导致 NFAT/AP-1 转录激活，这取决于细胞质尾部的 Y276^[2]。

Canine

HEK293

N-His

NP_001192043/XP_854888.1 (Y21-S160)

612069  [NCBI]

Partial

YVQVNGVVGHPATLPCTYSTASGVTTMCWGRGACPISHCLEEIVWTNGSHVTFQKHLRYKLKGKLSEGDVSLTIENAAQTDSGQYCCRVEHRGWFNDMKLTLSLEIKPDGNGTVTQSSDGLWHNNQTHVSLAQNSWMTTS

Approximately 32-40 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE. It migrates as an approximately 32-40 kDa band in SDS-PAGE under reducing conditions due to glycosylation.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.