TIM 蛋白, Human (His)

TIM protein is a highly efficient metabolic enzyme that catalyzes the rapid interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) during glycolysis and gluconeogenesis. Its key role in glucose metabolism is complemented by an additional responsibility: the production of methylglyoxal, a reactive cytotoxic by-product. TIM Protein, Human (His) is the recombinant human-derived TIM protein, expressed by E. coli , with N-6*His labeled tag. The total length of TIM Protein, Human (His) is 249 a.a., with molecular weight of ~30.0 kDa.

磷酸丙糖异构酶 (TIM) 是一种极其高效的代谢酶，对于催化糖酵解和糖异生的中心途径中磷酸二羟丙酮 (DHAP) 和 D-甘油醛-3-磷酸 (G3P) 之间的快速相互转化至关重要。除了在葡萄糖代谢中的关键作用之外，TIM 在甲基乙二醛（一种反应性和细胞毒性副产物）的生产中还承担着额外的责任。甲基乙二醛表现出修饰和诱导蛋白质、DNA 和脂质改变的潜力，强调了 TIM 参与基本代谢过程和细胞毒性副产物生成的双重性质。

Human

E. coli

N-6*His

P60174 (M1-Q249)

7167  [NCBI]

MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ

Approximately 30.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 1 mM DTT, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.