1. 重组蛋白
  2. Enzymes & Regulators
  3. Protease Inhibitors
  4. Tissue Inhibitor of Metalloproteinase (TIMPs)
  5. TIMP-1
  6. TIMP-1 蛋白, Human (HEK293)

TIMP-1 蛋白, Human (HEK293)

目录号: HY-P73597
COA 产品使用指南

TIMP-1 蛋白与目标金属蛋白酶形成一对一的复合物,通过与催化锌辅因子结合而不可逆地使其失活。它抑制多种 MMP,通过 CD63 和 ITGB1 激活细胞信号传导,并与 MMP1、MMP3、MMP10 和 MMP13 相互作用。TIMP-1 蛋白, Human (HEK293) 是重组的 TIMP-1 蛋白,由 HEK293 表达,不带标签。TIMP-1 蛋白, Human (HEK293) 全长 184 个氨基酸,分子量约为 ~26 kDa。

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规格 价格 是否有货 数量
20 μg ¥4500 In-stock
100 μg ¥12600 In-stock
> 100 μg   询价  

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描述

The TIMP-1 protein forms a one-to-one complex with the target metalloprotease, irreversibly inactivating it by binding to the catalytic zinc cofactor. It inhibits multiple MMPs, activates cell signaling through CD63 and ITGB1, and interacts with MMP1, MMP3, MMP10, and MMP13. TIMP-1 Protein, Human (HEK293) is the recombinant human-derived TIMP-1 protein, expressed by HEK293 , with tag free. The total length of TIMP-1 Protein, Human (HEK293) is 184 a.a., with molecular weight of ~26 kDa.

研究背景

TIMP-1 是一种金属蛋白酶抑制剂,通过与靶金属蛋白酶(包括胶原酶)形成一对一复合物来发挥作用,通过与催化锌辅因子结合导致其不可逆失活。其抑制谱包括 MMP1、MMP2、MMP3、MMP7、MMP8、MMP9、MMP10、MMP11、MMP12、MMP13 和 MMP16,不包括 MMP14。除了作为酶抑制剂的作用外,TIMP-1 还充当生长因子,协调细胞分化、迁移和细胞死亡,同时通过与 CD63 和 ITGB1 相互作用激活复杂的细胞信号级联。这种多面蛋白还在整合素信号传导中发挥着至关重要的作用,尤其是在体外介导红细胞生成,与 IL3 不同,对人类和小鼠红细胞祖细胞表现出物种特异性刺激。此外,TIMP-1 参与与 MMP1、MMP3、MMP10 和 MMP13 的蛋白质-蛋白质相互作用,证明其对这些金属蛋白酶的调节影响。它与 CD63 和 ITGB1 形成复合物,进一步强调其参与复杂的细胞信号网络。

体外研究
(In Vitro)

TIMP-1 (12.5-400 ng/mL;20 min) 增加 THP-1 单核细胞中 AKT 和 ERK1/2 的磷酸化水平。此外,TIMP-1 (100 ng/mL) 还能促进 THP-1 细胞中丝氨酸/苏氨酸激酶的激活[1]
TIMP-1 (50-200 ng/mL;1 h) 还能刺激 THP 细胞而介导细胞迁移和 CD74 内化,最佳浓度为 100 ng/mL[1]

生物活性

Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2 and the IC50 value is < 6 nM.

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P01033 (C24-A207)

基因 ID
蛋白结构
N-term
TIMP-1 (C24-A207)
Accession # P01033
C-term
同用名
Metalloproteinase Inhibitor 1; EPA; TIMP-1; CLGI; TIMP
分子量

Approximately 26 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM NaAC, 200 mM NaCl, pH 5.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TIMP-1 Protein, Human (HEK293)
目录号:
HY-P73597
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