1. 重组蛋白
  2. Enzymes & Regulators
  3. Protease Inhibitors
  4. Tissue Inhibitor of Metalloproteinase (TIMPs)
  5. TIMP-1
  6. TIMP-1 蛋白, Rat (HEK293)

TIMP-1 蛋白, Rat (HEK293)

目录号: HY-P73441
COA 产品使用指南

IgG3 Fc 蛋白是免疫球蛋白重链的恒定区,充当特定抗原的受体。TIMP-1 蛋白, Rat (HEK293) 是重组的 TIMP-1 蛋白,由 HEK293 表达,不带标签。TIMP-1 蛋白, Rat (HEK293) 全长 194 个氨基酸,分子量约为 ~28 kDa。

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规格 价格 是否有货 数量
20 μg ¥4250 In-stock
100 μg ¥11900 In-stock
> 100 μg   询价  

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描述

The IgG3 Fc protein is the constant region of an immunoglobulin heavy chain that acts as a receptor for a specific antigen. TIMP-1 Protein, Rat (HEK293) is the recombinant rat-derived TIMP-1 protein, expressed by HEK293 , with tag free. The total length of TIMP-1 Protein, Rat (HEK293) is 194 a.a., with molecular weight of ~28 kDa.

研究背景

免疫球蛋白重链的恒定区(称为抗体)代表 B 淋巴细胞产生的膜结合或分泌糖蛋白。在体液免疫的识别阶段,这些膜结合的免疫球蛋白充当受体,在与特定抗原结合后,触发 B 淋巴细胞克隆扩增并分化为分泌免疫球蛋白的浆细胞。分泌的免疫球蛋白在体液免疫的效应阶段发挥着关键作用,导致结合抗原的消除。抗原结合位点由一条重链的可变结构域与其相关轻链的可变结构域一起形成,导致每种免疫球蛋白具有两个对特定抗原具有显着亲和力的抗原结合位点。可变结构域经历 V-(D)-J 重排和随后的体细胞超突变,从而在暴露和选择后实现特定抗原的亲和力成熟。免疫球蛋白由两条相同的重链和两条相同的轻链组成,通过二硫键连接在一起。

生物活性

Measured by its ability to inhibit human MMP2 cleavage of a fluorogenic peptide substrate MCA-PLGLDPA-AR-NH2 and the IC50 value is approximately <4 nM.

种属

Rat

表达系统

HEK293

标签

Tag Free

蛋白编号

P30120 (C24-A217)

基因 ID

3502

蛋白结构
N-term
TIMP-1 (A1-A217)
Accession # P01860
C-term
同用名
Metalloproteinase Inhibitor 1; EPA; TIMP-1; CLGI; TIMP
氨基酸序列

ASTKGPSVFPLAPCSRSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYTCNVNHKPSNTKVDKRVELKTPLGDTTHTCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPEPKSCDTPPPCPRCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVQFKWYVDGVEVHNA

分子量

Approximately 28 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TIMP-1 Protein, Rat (HEK293)
目录号:
HY-P73441
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