TIMP-2 蛋白, Mouse (HEK293, His)

TIMP-2 is a tissue inhibitor of metalloproteinases that forms complexes with metalloproteinases, especially collagenases, effectively and irreversibly inactivating them by binding to their catalytic zinc cofactor. This interaction plays a crucial role in regulating the activity of metalloproteinases. TIMP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived TIMP-2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of TIMP-2 Protein, Mouse (HEK293, His) is 194 a.a., with molecular weight of 21-23 kDa.

金属蛋白酶组织抑制剂 2 (TIMP-2) 是一种与金属蛋白酶（包括胶原酶）形成复合物的蛋白质，通过与其催化锌辅因子结合，导致这些酶不可逆失活。值得注意的是，TIMP-2 通过其 C 末端区域与基质金属蛋白酶 2 (MMP2) 表现出特异性相互作用。这种相互作用，特别是与 MMP2 的 C 端 PEX 结构域的相互作用，导致 MMP2 活性的抑制。TIMP-2 调节金属蛋白酶的能力强调了其在控制细胞外基质重塑中的重要性，并对各种生理和病理过程产生影响。（参考：提供的资料）

Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The IC₅₀ is 4.79 nM, as measured under the described conditions.

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  Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ this effect is 34.88 ng/mL, corresponding to a specific activity is 2.87×10⁴ units/mg.

Mouse

HEK293

C-6*His

P25785 (C27-P220)

21858  [NCBI]

CSCSPVHPQQAFCNADVVIRAKAVSEKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPDKDIEFIYTAPSSAVCGVSLDVGGKKEYLIAGKAEGDGKMHITLCDFIVPWDTLSITQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMDWVTEKSINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP

21-23 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.