1. 重组蛋白
  2. Cytokines and Growth Factors Biotinylated Proteins
  3. TNF Superfamily Neurotrophic Factors
  4. TNF Superfamily Ligands
  5. TNF-alpha
  6. TNF-alpha/TNFSF2 蛋白, Human (HEK293, His-Avi)

TNF-alpha/TNFSF2 蛋白, Human (HEK293, His-Avi)

目录号: HY-P78527
COA 产品使用指南

TNF alpha (肿瘤坏死因子 α) 是一种由 157 个氨基酸组成的同源三聚体蛋白,是一种有效的促炎细胞因子。TNF alpha 与其受体结合,主要是 TNFR1 和 TNFR2 ,然后传递信号以实现炎症和细胞死亡等生物学功能。TNF alpha 通过 TNFR2 受体激活 NF-κB 通路促进癌细胞生长、侵袭和转移,但抗 TNF-α 抗体能显着抑制结肠炎相关癌症 (CAC) 小鼠的肿瘤发展。TNF alpha 作为一种原神经原因子可激活 SAPK/JNK 通路,并能够促进神经元替代和脑修复以应对脑损伤。TNF-alpha/TNFSF2 蛋白, Human (Biotinylated, HEK293, His-Avi) 是一种由 HEK293 细胞表达的生物素化的重组蛋白,其 C 末端带有一个 Avi 标签和一个 His 标签,由 157 个 (V77-L233) 氨基酸组成。

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规格 价格 是否有货 数量
20 μg ¥1780 In-stock
50 μg ¥3750 In-stock
100 μg   询价  
250 μg   询价  

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描述

Tumour Necrosis Factor alpha (TNF alpha) is a homotrimer protein consisting of 157 amino acids, is a potent pro-inflammatory cytokine[1]. TNF alpha binds to its receptors, mainly TNFR1 and TNFR2, and then transmits molecular signals for biological functions such as inflammation and cell death[3]. TNF alpha stimulates NF-κB pathway via TNFR2 promotes cancer growth, invasion, and metastasis. Anti-TNF-α MAb significantly suppresses the tumor development in colitis-associated cancer (CAC) mouse[5]. TNF alpha as a proneurogenic factor activates the SAPK/JNK Pathway and can facilitate neuronal replacement and brain repair in response to brain injury[6]. TNF-alpha/TNFSF2 Protein, Human (HEK293, His-Avi) is a recombinant protein with a C-Terminal Avi and a His label, It consists of 157 amino acids (V77-L233) and is produced in HEK293 cells.

研究背景

TNF alpha is produced by various types of cells including macrophages, monocytes, neutrophils, T cells, and NK-cells[2].
The amino acid sequence of human TNF alpha protein has low homology between mouse, rat, bovine, cynomolgus TNF alpha protein. While, human TNF alpha shares 94.85% aa sequence identity with cynomolgus TNF alpha protein, mouse TNF alpha shares 94.47% aa sequence identity with rat TNF alpha protein.
TNF alpha exists in two forms; a type II transmembrane protein (tmTNF-α) and a mature soluble protein (sTNF-α). TNF-α binds to its receptors, mainly TNFR1 and TNFR2, and then transmits molecular signals for biological functions such as inflammation and cell death. Both sTNF-α and tmTNF-α activate TNFR1, and process a death domain (DD) that interacts with the TNFR1-associated death domain (TRADD) adaptor protein. The TNFR2 signaling pathway is mainly activated by tmTNF-α. TNFR1 signaling tends to be pro-inflammatory and apoptotic. TNFR2 results in NF-κB and MAPKs and AKT activation, TNFR2 activation is associated with homeostatic bioactivities such as tissue regeneration, cell proliferation, and cell survival, as well as host defense and inflammation[1].
TNF-alpha is critical for normal immune response, abnormal secretion TNF alpha activates synovial fibroblasts, keratinocytes, osteoclasts, induces rheumatoid arthritis, inflammatory bowel disease, psoriatic arthritis (PsA), and noninfectious uveitis (NIU)[3]. TNF alpha positively regulates endogenous TNF-α expression levels independently of Pgp efflux activity, induces IHF cells proliferation[4]. TNF alpha in tissues may promote cancer growth, invasion, and metastasis. Besides, TNF alpha stimulates NF-κB pathway via TNFR2 and anti-TNF-α MAb significantly suppresses the tumor development in colitis-associated cancer (CAC) mouse[5]. TNF alpha as a proneurogenic factor activates the SAPK/JNK pathway and can facilitate neuronal replacement and brain repair in response to brain injury[6].

体外研究
(In Vitro)

TNF alpha (human) (0, 10, 15, 20, 30, 50 ng/mL; 24, 48 h) reduces KB-C1 cells viability at 30 ng/mL after 48 h, reduces pro-caspase-3, cleaved caspase-3 expression in KB-C1 and KB-3-1 cells[4].
TNF alpha (human) (10, 15 ng/mL; 24 h) significantly increases the mRNA levels of Pgp (ABCB1) in KB-3-1 cells, and promotes expressive reduction on total Pgp protein levels and a slightly decreases in cell surface-Pgp in KB-C1 cells, and stimulates IHF cells proliferation, probably via ERK activation[4].

体内研究
(In Vivo)

TNF alpha (human) (100 µg; osmium pump in back; daily for 7 days) significantly increases in the number of inflammatory cells and the degree of synovial inflammation is significantly exacerbated in twenty-four SCID‐HuRAg mice[7].

生物活性

Immobilized Human TNF alpha, His Tag at 5 ug/ml (100 μl/Well) on the plate. Dose response curve for HHuman TNFR2, hFc Tag with the EC50 of 88 ng/ml determined by ELISA.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

P01375 (V77-L233)

中文名
重组人 TNF-alpha/TNFSF17 蛋白
同用名
APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2
分子量

21-25 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

Lyophilized powder.

组分

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5 % trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TNF-alpha/TNFSF2 Protein, Human (HEK293, His-Avi)
目录号:
HY-P78527
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