TNFR-1/CD120a 蛋白, Human (HEK293, Fc)

The TNFR-1/CD120a protein is a receptor for TNFSF2/TNF-α and TNFSF1/lymphotoxin-α, and initiates caspase-8-mediated apoptosis upon TNF binding. It induces non-cytocidal TNF action, activates acid sphingomyelinase and establishes an antiviral state. TNFR-1/CD120a Protein, Human (HEK293, Fc) is the recombinant human-derived TNFR-1/CD120a protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TNFR-1/CD120a Protein, Human (HEK293, Fc) is 182 a.a., with molecular weight of 55-70 kDa.

TNFR-1/CD120a 蛋白充当 TNFSF2/TNF-α 和同源三聚体 TNFSF1/淋巴毒素-α 的受体。TNF 结合后，接头分子 FADD 将 caspase-8 招募到激活的受体上，启动死亡诱导信号复合物 (DISC) 的形成。该复合物导致 caspase-8 蛋白水解激活，引发随后的 caspase 介导的细胞凋亡。TNFR-1/CD120a 参与诱导非杀细胞性 TNF 效应，包括抗病毒状态的建立和酸性鞘磷脂酶的激活。TNFR-1/CD120a 与 TNF 结合后的同源三聚化提供了与 TRADD 死亡结构域特异性相互作用的分子界面，招募各种 TRADD 相互作用蛋白，如 TRAFS、RIPK1 和可能的 FADD。该复合物激活不同的信号级联，包括细胞凋亡和 NF-kappa-B 信号传导。此外，TNFR-1/CD120a 与多种蛋白相互作用，包括 BAG4、BABAM2、FEM1B、GRB2、SQSTM1、TRPC4AP、NOL3、SH3RF2、PGLYRP1 和 MADD，在调节 TNF 信号通路和诱导细胞凋亡中发挥作用。

Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity inthe L-929 mouse fibroblast cells in the presence of the metabolic inhibitoractinomycin D.The ED₅₀ for this effect is 0.1558 μg/mLin the presence of 0.25 ng/mL of recombinant humanTNF-alpha, corresponding to a specific activity is6.418×10³ units/mg.

Human

HEK293

C-hFc

P19438 (L30-T211)

7132  [NCBI]

LVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTT

55-70kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.