TNF RI/TNFRSF1A 蛋白, Human (HEK293, His)

The TNFR-1/CD120a protein is a receptor for TNFSF2/TNF-α and TNFSF1/lymphotoxin-α, and initiates caspase-8-mediated apoptosis upon TNF binding. It induces non-cytocidal TNF action, activates acid sphingomyelinase and establishes an antiviral state. TNFR-1/CD120a Protein, Human (HEK293, His) is the recombinant human-derived TNFR-1/CD120a protein, expressed by HEK293 , with C-8*His labeled tag.

TNFRSF1A (TNF RI) 蛋白是一种单通道 I 型膜蛋白，属于肿瘤坏死因子 (TNF) 家族。TNFRSF1A 是 TNF-α 的主要信号受体。TNFRSF1A蛋白是一种多功能细胞因子，几乎所有细胞均合成该蛋白^[1][2]。
不同物种TNFRSF1A的氨基酸序列为非常不同（人类、大鼠和小鼠之间的相似性低于 85%）。
TNFRSF1A 包含一个蛋白质-蛋白质相互作用结构域，称为死亡结构域 (DD)，可以招募其他含有 DD 的蛋白质并将死亡受体与 caspase 偶联激活和凋亡。可溶性和膜结合形式的细胞因子均可激活 TNFRSF1A。TNFRSF1A 通过参与 mTOR、JNK、IKK、caspase 3、MAPK 和 NF-κB 通路诱导细胞炎症损伤和细胞凋亡^[1][3][4]。

1.Measured  by its ability to inhibit the TNF-alpha mediated cytotoxicity inthe L-929  mouse fibroblast cells in the presence of the metabolic inhibitoractinomycin  D.The ₅₀ for this effect is ≤0.06164 μg/mLin the presence of 0.25 ng/mL of  recombinant humanTNF-alpha, corresponding to a specific activity is ≥1.622×10⁴ units/mg.
2.Anti-His antibody Immobilized on CM5 Chip captured TNFR-1/CD120a His Tag, Human, can bind TNF-α, Human with an affinity constant of 0.106 nM as determined in SPR assay.

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  Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity inthe L-929 mouse fibroblast cells in the presence of the metabolic inhibitoractinomycin D.The ED₅₀ for this effect is 0.06164 μg/mLin the presence of 0.25ng/mL of recombinant humanTNF-alpha, corresponding to a specific activity is 1.622×10⁴ units/mg.

Human

HEK293

C-8*His

P19438-1 (L30-T211)

7132  [NCBI]

Extracellular Domain

LVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTT

28-38kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.