TPM1 蛋白, Human (His)

TPM1 encodes tropomyosin, a key actin-binding component in muscle contraction and cytoskeletal structure. It contains α-helical chains that form a coiled-coil structure that stabilizes actin filaments. TPM1 Protein, Human (His) is the recombinant human-derived TPM1 protein, expressed by E. coli , with N-His labeled tag. The total length of TPM1 Protein, Human (His) is 284 a.a., with molecular weight of ~35 kDa.

TPM1 蛋白是原肌球蛋白家族的成员，属于一组高度保守的肌动蛋白结合蛋白，对于横纹肌和平滑肌的收缩系统以及非肌肉细胞的细胞骨架至关重要。原肌球蛋白由两条排列成卷曲螺旋的 α 螺旋链组成，沿着肌动蛋白丝的凹槽聚合，提供结构稳定性。这种蛋白质是关键的 α 螺旋链，是横纹肌中主要的原肌球蛋白，与肌钙蛋白复合物合作调节肌肉收缩过程中肌动蛋白和肌球蛋白之间的钙依赖性相互作用。在平滑肌和非肌肉细胞中，选择性剪接产生各种同种型。TPM1 基因突变与 3 型家族性肥厚型心肌病和 1Y 型扩张型心肌病相关。此外，TPM1 显示出偏向表达，在心脏和前列腺以及其他九种组织中观察到显着水平，强调了其在不同生理环境中的重要性。

Human

E. coli

N-His

P09493-10/NP_000357.3 (M1-M284)

7168  [NCBI]

MDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQEKLELAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGQVRQLEEQLRIMDQTLKALMAAEDKYSQKEDRYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSM

Approximately 35 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.