TPM3 蛋白, Human

TPM3 is a member of the tropomyosin family that binds actin filaments in muscle and nonmuscle cells. In striated muscle, it regulates calcium-dependent contraction together with the troponin complex. TPM3 Protein, Human is the recombinant human-derived TPM3 protein, expressed by E. coli , with tag free.

TPM3 蛋白是原肌球蛋白家族的成员，在与肌肉和非肌肉细胞中的肌动蛋白丝结合方面表现出多种功能。TPM3 与肌钙蛋白复合物结合，在脊椎动物横纹肌收缩的钙依赖性调节中发挥着核心作用。它参与平滑肌收缩是通过与卡尔德斯蒙相互作用介导的。除了肌肉相关功能之外，TPM3 还参与稳定非肌肉细胞的细胞骨架内的肌动蛋白丝。在结构上，TPM3 与 α（TPM1、TPM3 或 TPM4）和 β（TPM2）链结合形成同二聚体和异二聚体。此外，TPM3 与 TMOD1 相互作用，进一步发挥其在跨各种细胞环境调节肌动蛋白动力学方面的多方面作用。

Human

E. coli

Tag Free

P06753-2 (M1-M248)

7170  [NCBI]

Full Length of Isoform-2

MAGITTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEGDLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKLKEAETRAEFAERSVAKLEKTIDDLEDKLKCTKEEHLCTQRMLDQTLLDLNEM

Approximately 20.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.