1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. TPP1 蛋白, Mouse (sf9, His)

TPP1 蛋白, Mouse (sf9, His)

目录号: HY-P76112
产品使用指南

TPP1 是一种溶酶体丝氨酸蛋白酶,作为一种非特异性溶酶体肽酶,在溶酶体蛋白降解中表现出三肽基肽酶 I 活性。其功能包括从其他溶酶体蛋白酶的分解产物中产生三肽,强调其在溶酶体环境中加工和水解肽中的作用。值得注意的是,TPP1 需要具有未取代的 N 末端的底物。TPP1 蛋白, Mouse (sf9, His) 是重组的 TPP1 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。TPP1 蛋白, Mouse (sf9, His) 全长 562 个氨基酸,分子量约为 ~60.9 kDa。

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描述

TPP1, a lysosomal serine protease, acts as a non-specific lysosomal peptidase, displaying tripeptidyl-peptidase I activity in lysosomal protein degradation. Its function involves generating tripeptides from the breakdown products of other lysosomal proteinases, emphasizing its role in processing and hydrolyzing peptides within the lysosomal environment. Notably, TPP1 requires substrates with an unsubstituted N-terminus. TPP1 Protein, Mouse (sf9, His) is the recombinant mouse-derived TPP1 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of TPP1 Protein, Mouse (sf9, His) is 562 a.a., with molecular weight of ~60.9 kDa.

研究背景

TPP1 是一种溶酶体丝氨酸蛋白酶,具有三肽基肽酶 I 活性,其参与溶酶体蛋白质降解就证明了这一点。虽然其精确的底物特异性尚未完全阐明,但 TPP1 被认为具有非特异性溶酶体肽酶的功能,从其他溶酶体蛋白酶产生的分解产物中产生三肽。值得注意的是,TPP1 需要具有未取代的 N 末端的底物,这进一步凸显了它在溶酶体环境中加工和水解肽中的作用。

种属

Mouse

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

O89023 (M1-P562)

基因 ID

12751  [NCBI]

蛋白结构
N-term
TPP1 (M1-P562)
Accession # O89023
His
C-term
同用名
Tripeptidyl-Peptidase 1; LPIC; Tripeptidyl Aminopeptidase; TPP-I; CLN2
分子量

Approximately 60.9 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TPP1 Protein, Mouse (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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