1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens
  3. TNF Superfamily NK Cell CD Proteins
  4. TNF Superfamily Ligands CD253/TRAIL
  5. TRAIL Proteins
  6. TRAIL/TNFSF10 蛋白, Human

TRAIL/TNFSF10 蛋白, Human

目录号: HY-P7306
COA 产品使用指南

TRAIL 蛋白 (TNFSF10),TNF 超家族的成员,是一种 II 型跨膜蛋白。TRAIL 蛋白主要与 TRAIL-R1 和 TRAIL-R2 两种受体相互作用,并诱导肿瘤或感染细胞凋亡。TRAIL 蛋白还可与 DR4、DR5 和 OPG 三种受体结合。当与 DR4 或 DR5 结合时,TRAIL 蛋白可以募集 FADD 并进一步募集和激活 caspase-8。此外,TRAIL 还可能通过激活促炎通路触发非凋亡信号通路。TRAIL 蛋白主要表达于免疫细胞表面,如细胞毒性 T 细胞和自然杀伤 (NK) 细胞。TRAIL/TNFSF10 蛋白, Human 是一种重组人 TRAIL (V114-G281),无标签,在 E. coli 中表达。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥1100 In-stock
50 μg ¥2500 In-stock
100 μg   询价  
250 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

TRAIL Protein (TNFSF10), a member of the TNF superfamily, is a type II transmembrane protein. TRAIL Protein mainly interacts with TRAIL-R1 and TRAIL-R2, and induces apoptosis in tumor or infected cells. TRAIL Protein also binds with DR4, DR5, and OPG. TRAIL Protein can recruit FADD and further recruit and activates caspase-8 after binding to DR4 or DR5. Besides, TRAIL may also trigger nonapoptotic signaling through activating pro-inflammatory pathways[1][2]. TRAIL protein is mainly expressed on surface of immune cells, such as cytotoxic T cells and natural killer (NK) cell[1]. TRAIL/TNFSF10 Protein, Human is a recombinant human TRAIL (V114-G281) without any tag, which is expressed in E. coli.

研究背景

TRAIL Protein (TNFSF10), a member of the TNF superfamily, is a type II transmembrane protein. TRAIL Protein is expressed in various tissues, especially in the spleen, lung, and prostate. TRAIL protein is mainly expressed on surface of immune cells, such as cytotoxic T cells and natural killer (NK) cell. TRAIL proteins on NK and T cells is critical for controlling virus infections and tumor immune surveillance[1][2].
Human TRAIL consists of cytoplasmic domain (M1-V17), helical domain (L18-F38), and extracellular domain (T39-G281). Human TRAIL Protein shares < 70% common aa identity with mouse and rat. Mouse TRAIL Protein shares 86.94% common aa identity with rat.
TRAIL Protein mainly interacts with two agonistic TRAIL receptors (TRAIL-R1 and TRAIL-R2) and induces apoptosis in tumor or infected cells. TRAIL Protein also binds with DR4, DR5, and OPG. When binding to DR4 or DR5, TRAIL Protein can recruit FADD and further recruit and activates caspase-8. Besides, TRAIL may also trigger nonapoptotic signaling through activating pro-inflammatory pathways, such as NF-κB, PI3K/Akt, and MAPK pathway[1][2].
TRAIL induces apoptosis of tumor cells in a p53 independent manner. TRAIL-based therapies has high anti-tumor potential[3].

体外研究
(In Vitro)

TRAIL (human, 10 ng/mL-10 μg/mL, 24 h) reduces cell viability of HepG2 cells[4].
TRAIL (human, 1-10 ng, 24 h) induces pathognomic features of asthma and stimulates the production of CCL20 in primary human bronchial epithelium cells[5].

生物活性

The ED50 is <40 ng/mL as measured by RPMI-8226 cells, corresponding to a specific activity of >2.5 × 104 units/mg.

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P50591 (V114-G281)

基因 ID

8743  [NCBI]

中文名
重组人 TRAIL/TNFSF10 蛋白
同用名
rHuTRAIL/Apo2L; TNFSF10; CD253
氨基酸序列

MVRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG

分子量

Approximately 20 kDa

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
TRAIL/TNFSF10 Protein, Human
目录号:
HY-P7306
需求量: