1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TNF Superfamily
  4. TNF Receptor Superfamily
  5. Death Receptor 5
  6. TRAIL R2/TNFRSF10B 蛋白, Human (127a.a, HEK293, His)

TRAIL R2/TNFRSF10B 蛋白, Human (127a.a, HEK293, His)

目录号: HY-P70806
COA 产品使用指南

TRAIL R2/TNFRSF10B 蛋白是 TNFSF10/TRAIL 的受体,通过 FADD 招募 caspase-8 来启动细胞凋亡。它形成死亡诱导信号复合物 (DISC),激活半胱天冬酶并介导细胞凋亡。TRAIL R2/TNFRSF10B 蛋白, Human (127a.a, HEK293, His) 是重组的 TRAIL R2/TNFRSF10B 蛋白,由 HEK293 表达,带有 C-6*His 标签。TRAIL R2/TNFRSF10B 蛋白, Human (127a.a, HEK293, His) 全长 127 个氨基酸,分子量约为 ~18.0 kDa。

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描述

TRAIL R2/TNFRSF10B protein is the receptor of TNFSF10/TRAIL and recruits caspase-8 through FADD to initiate cell apoptosis. It forms the death-inducing signaling complex (DISC), activates caspases and mediates apoptosis. TRAIL R2/TNFRSF10B Protein, Human (127a.a, HEK293, His) is the recombinant human-derived TRAIL R2/TNFRSF10B protein, expressed by HEK293 , with C-6*His labeled tag. The total length of TRAIL R2/TNFRSF10B Protein, Human (127a.a, HEK293, His) is 127 a.a., with molecular weight of ~18.0 kDa.

研究背景

TRAIL R2/TNFRSF10B 蛋白作为细胞毒性配体 TNFSF10/TRAIL 的受体发挥作用。配体结合后,接头分子 FADD 将 caspase-8 募集到激活的受体上,形成死亡诱导信号复合物 (DISC),从而触发 caspase-8 蛋白水解激活并启动随后的 caspase 级联,介导细胞凋亡。此外,TRAIL R2/TNFRSF10B 促进 NF-kappa-B 的激活,对于内质网 (ER) 应激诱导的细胞凋亡至关重要。在单体状态下,它可以与 TRADD 和 RIPK1 相互作用,在没有刺激的情况下,它可以与 BIRC2、DDX3X 和 GSK3B 相互作用。受体的刺激增强了与 BIRC2 和 DDX3X 的相互作用,并伴随着它们的裂解。值得注意的是,TRAIL R2/TNFRSF10B 还可以与 HCMV 蛋白 UL141 相互作用,防止细胞表面表达,其中两个 TNFRSF10B 单体与 UL141 同二聚体相互作用,三个 TNFRSF10B 分子与 TNFSF10 同三聚体相互作用。这些复杂的相互作用强调了 TRAIL R2/TNFRSF10B 在细胞凋亡和信号传导途径中的多方面作用。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

O14763 (I56-E182)

基因 ID
蛋白结构
N-term
TRAIL-R2 (I56-E182)
Accession # O14763
6*His
C-term
同用名
Tumor Necrosis Factor Receptor Superfamily Member 10B; Death Receptor 5; TNF-Related Apoptosis-Inducing Ligand Receptor 2; TRAIL Receptor 2; TRAIL-R2; CD262; TNFRSF10B; DR5; KILLER; TRAILR2; TRICK2; ZTNFR9
氨基酸序列

ITQQDLAPQQRAAPQQKRSSPSEGLCPPGHHISEDGRDCISCKYGQDYSTHWNDLLFCLRCTRCDSGEVELSPCTTTRNTVCQCEEGTFREEDSPEMCRKCRTGCPRGMVKVGDCTPWSDIECVHKE

分子量

Approximately 18.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

TRAIL R2/TNFRSF10B Protein, Human (127a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TRAIL R2/TNFRSF10B Protein, Human (127a.a, HEK293, His)
目录号:
HY-P70806
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