Transglutaminase 3/TGM3 蛋白, Human (sf9, His)

Transglutaminase 3 (TGM3) serves as a catalyst in the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in diverse proteins, alongside mediating the conjugation of polyamines to proteins. This enzymatic activity plays a crucial role in the intricate process of cornified envelope (CE) formation, a specialized structure comprising covalent cross-links of proteins beneath the plasma membrane in fully differentiated keratinocytes. TGM3 is actively involved in cross-linking small proline-rich proteins (SPRR1 and SPRR2) and loricrin (LOR) to create small interchain oligomers. These oligomers are subsequently cross-linked by TGM1 onto the developing CE scaffold, contributing to the structural integrity of this vital component. Notably, TGM3's involvement extends to hair follicles, where it participates in cross-linking structural proteins to fortify the inner root sheath. Transglutaminase 3/TGM3 Protein, Human (sf9, His) is the recombinant human-derived Transglutaminase 3/TGM3 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Transglutaminase 3/TGM3 Protein, Human (sf9, His) is 692 a.a., with molecular weight of ~70 kDa.

转谷氨酰胺酶 3 (TGM3) 在多种蛋白质中的谷氨酰胺和赖氨酸残基之间钙依赖性异肽交联形成中充当催化剂，同时介导多胺与蛋白质的缀合。这种酶活性在角质化包膜 (CE) 形成的复杂过程中起着至关重要的作用，角质化包膜是一种特殊的结构，由完全分化的角质形成细胞质膜下方的蛋白质共价交联组成。 TGM3 积极参与富含脯氨酸的小蛋白（SPRR1 和 SPRR2）和 loricrin (LOR) 的交联，以产生小的链间寡聚物。这些寡聚物随后通过 TGM1 交联到正在开发的 CE 支架上，从而有助于这一重要成分的结构完整性。值得注意的是，TGM3 的参与延伸到毛囊，它参与交联结构蛋白以强化内根鞘。

Measured by its ability to cleave a synthetic peptide Benzvloxvcarbonvl-GIn-Glvand NH2OH.The specific activity is 1018.3 pmol/min/μg, as measured under the described conditions.

Human

Sf9 insect cells

N-His

Q08188 (A2-E693)

7053  [NCBI]

Approximately 78 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 10% gly

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.