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Transthyretin/TTR 蛋白, Human (127a.a, HEK293, His)

目录号: HY-P70500
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Transthyretin (TTR) 属于血清转运蛋白家族,是 T4 和视黄醇的血浆运输载体。Transthyretin 抑制 Aβ1-42 纤维化,是淀粉样变性和阿尔茨海默病的潜在靶点。Transthyretin 突变 (如 V30M) 可破坏四聚体稳定性,促使单体形成淀粉样纤维引发遗传性淀粉样变性转甲状腺素蛋白 (ATTRv)。Transthyretin/TTR 蛋白, Human (127a.a, HEK293, His) 是重组的运甲状腺素蛋白/TTR 蛋白,由 HEK293 表达,带有 C-6*His 标签。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

Transthyretin (TTR) belongs to the serum transporter family and is a plasma transporter of T4 and retinol. Transthyretin inhibits Aβ1-42 fibrillization and is a potential target for amyloidosis and Alzheimer's disease. Transthyretin mutations (such as V30M) can destabilize the tetramer and promote the formation of amyloid fibrils in monomers, causing hereditary amyloidosis transthyretin (ATTRv). Transthyretin/TTR Protein, Human (127a.a, HEK293, His) is a recombinant transthyretin/TTR protein expressed in HEK293 with a C-6*His tag.

研究背景

1. Transthyretin/TTR 的蛋白特点
Transthyretin (TTR) 是通过二硫键连接的同源四聚体,含保守的 β-折叠结构及两个甲状腺素 (T4) 结合位点。Transthyretin/TTR 需经信号肽切割后形成成熟蛋白,通过 T4 结合维持四聚体稳定性[1]。Transthyretin/TTR 属于血清转运蛋白家族,是 T4 和视黄醇的血浆运输载体,与视黄醇结合蛋白形成复合物,介导维生素 A 运输,维持甲状腺激素和维生素 A 的稳态[2]。而突变 (如 V30M、L55P),可破坏 Transthyretin/TTR 四聚体稳定性,促使单体解离并自组装为 β-片层纤维,沉积于神经、心脏等组织引发遗传性转甲状腺素蛋白淀粉样变性 (ATTRv)。非纤维寡聚体通过钙内流及内质网应激导致细胞毒性[1]
Transthyretin/TTR 与 Aβ1-42 结合,通过 T4 结合口袋抑制其纤维化,延缓阿尔茨海默病相关病理[2]。或 Transthyretin/TTR 与糖胺聚糖等内源性因子协同促进淀粉样沉积[1]

2. Transthyretin/TTR 的应用
Transthyretin/TTR 可用于淀粉样变性疾病 ATTRv 的病理机制研究。如构建突变体 (如 L55P) 转基因小鼠用于药物筛选,研究 Transthyretin/TTR 稳定剂,如 Diflunisal 通过稳定 Transthyretin/TTR 四聚体延缓淀粉样纤维形成[3];同时,稳定剂 Tafamidis 已被证实可用于抑制 ATTRv 神经病[1]。Transthyretin/TTRR 靶向 Aβ 聚集,是阿尔茨海默病的潜在靶点[2]

体外研究
(In Vitro)

Transthyretin/TTR Protein (人源 huTTR;0.25-2 μM) 在硫黄素 T (ThT,20 μM) 荧光测定中抑制 Aβ1-40 聚合纤维 (10 μM) 形成,其中突变体 M-TTR (F87M/L110M) 抑制效果最强,WT huTTR 次之,鼠源 muTTR 最弱[2]
Transthyretin/TTR Protein (人源 huTTR;2.5 μM;5 d) 抑制 Aβ1-42 的纤维和寡聚体形成,突变体 M-TTR 处理组无明显纤维结构,huTTR 组纤维数量减少,muTTR 组无显著抑制[2]
Transthyretin/TTR Protein (人源 huTTR;20 μM;3 min) 与固定化 Aβ1-40 单体结合,结合强度排序为 M-TTR>V30M TTR≥WT huTTR>V122I TTR[2]
Transthyretin/TTR Protein (

生物活性

Measured by its binding ability in a functional ELISA. Immobilized recombinant human TTR-His at 10 μg/ml (100 μl/well) can bind recombinant Canine RBP4-Fc with a linear range of 0.3-10.0 μg/ml.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P02766 (G21-E147)

基因 ID
蛋白结构
N-term
TTR (G21-E147)
Accession # P02766
6*His
C-term
蛋白长度

Full Length of Mature Protein

中文名
Transthyretin 蛋白/TTR 蛋白
同用名
Transthyretin; ATTR; Prealbumin; TBPA; TTR; PALB
氨基酸序列

GPTGTGESKCPLMVKVLDAVRGSPAINVAVHVFRKAADDTWEPFASGKTSESGELHGLTTEEEFVEGIYKVEIDTKSYWKALGISPFHEHAEVVFTANDSGPRRYTIAALLSPYSYSTTAVVTNPKE

预测分子量
15 kDa
分子量

Approximately 18 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

糖基化修饰
Yes
构象/形态
Homotetramer
纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.8.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.
4.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Transthyretin/TTR Protein, Human (127a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Transthyretin/TTR Protein, Human (127a.a, HEK293, His)
目录号:
HY-P70500
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