TRIM21 蛋白, Mouse (His-SUMO)

TRIM21 is an E3 ubiquitin protein ligase that forms complexes with E2 enzymes including UBE2D1 and UBE2E2. It cooperates with UBE2D2 to ubiquitinate USP4, IKBKB and itself, and binds to SCF E3 ligase to mediate ubiquitination of CDKN1B. TRIM21 Protein, Mouse (His-SUMO) is the recombinant mouse-derived TRIM21 protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of TRIM21 Protein, Mouse (His-SUMO) is 470 a.a., with molecular weight of ~70.2 kDa.

TRIM21 蛋白作为 E3 泛素蛋白连接酶发挥作用，依赖于 E2 酶（UBE2D1、UBE2D2、UBE2E1、UBE2E2）。它与 UBE2D2 形成泛素连接酶复合物，泛素化 USP4、IKBKB 及其自身。它与基于 cullin-RING 的 SCF E3 连接酶复合物相关，介导 CDKN1B 泛素化并参与 Tax 诱导的 NF-kappa-B 信号传导的负调节。TRIM21 负向调节 IFN-β 的产生并介导泛素介导的 IgG1 重链降解。它促进 IRF8 泛素化，影响细胞周期进程，增强 DCP2 脱帽活性，并在自噬调节中发挥作用。此外，TRIM21 还参与先天免疫、炎症反应和抗病毒防御，介导影响细胞过程的泛素化事件。

Mouse

E. coli

N-His;N-SUMO

Q62191 (1M-470M)

20821  [NCBI]

MSPSTTSKMSLEKMWEEVTCSICLDPMVEPMSIECGHCFCKECIFEVGKNGGSSCPECRQQFLLRNLRPNRHIANMVENLKQIAQNTKKSTQETHCMKHGEKLHLFCEEDGQALCWVCAQSGKHRDHTRVPIEEAAKVYQEKIHVVLEKLRKGKELAEKMEMDLTMQRTDWKRNIDTQKSRIHAEFALQNSLLAQEEQRQLQRLEKDQREYLRLLGKKEAELAEKNQALQELISELERRIRGSELELLQEVRIILERSGSWNLDTLDIDAPDLTSTCPVPGRKKMLRTCWVHITLDRNTANSWLIISKDRRQVRMGDTHQNVSDNKERFSNYPMVLGAQRFSSGKMYWEVDVTQKEAWDLGVCRDSVQRKGQFSLSPENGFWTIWLWQDSYEAGTSPQTTLHIQVPPCQIGIFVDYEAGVVSFYNITDHGSLIYTFSECVFAGPLRPFFNVGFNYSGGNAAPLKLCPLKM

Approximately 70.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in Tris-based buffer, 50% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.