1. 重组蛋白
  2. Receptor Proteins Enzymes & Regulators
  3. Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Trk Receptors TrkA
  5. TrkA 蛋白, Human (HEK293, His-Fc)

TrkA 蛋白, Human (HEK293, His-Fc)

目录号: HY-P73458
产品使用指南

TrkA蛋白是一种重要的受体酪氨酸激酶,调节中枢和周围神经系统的发育,影响神经元的增殖、分化和存活。作为 NGF 的高亲和力受体,TrkA 在 NGF 结合后通过同二聚化和自磷酸化被激活。TrkA 蛋白, Human (HEK293, His-Fc) 是重组的 TrkA 蛋白,由 HEK293 表达,带有 C-hFc, C-His 标签。TrkA 蛋白, Human (HEK293, His-Fc) 全长 382 个氨基酸,分子量为 85-95 kDa。

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描述

TrkA protein is an important receptor tyrosine kinase that regulates the development of the central and peripheral nervous systems and affects the proliferation, differentiation, and survival of neurons. As a high-affinity receptor for NGF, TrkA is activated upon NGF binding through homodimerization and autophosphorylation. TrkA Protein, Human (HEK293, His-Fc) is the recombinant human-derived TrkA protein, expressed by HEK293 , with C-hFc, C-His labeled tag. The total length of TrkA Protein, Human (HEK293, His-Fc) is 382 a.a., with molecular weight of 85-95 kDa.

研究背景

TrkA 蛋白是一种受体酪氨酸激酶,在中枢和周围神经系统的发育和成熟中发挥着至关重要的作用,控制交感神经元和感觉神经元的增殖、分化和存活。TrkA 作为 NGF(其主要配体)的高亲和力受体发挥作用,在二聚体 NGF 配体结合后经历同二聚化、自磷酸化和激活。这种激活导致下游效应子(包括 SHC1、FRS2、SH2B1、SH2B2 和 PLCG1)的募集、磷酸化和/或激活,协调驱动细胞存活和分化的不同信号级联。TrkA 通过 GRB2-Ras-MAPK 级联、Ras-PI3 激酶-AKT1 信号传导和 NF-Kappa-B 激活等途径调节多种细胞过程。在没有配体和激活的情况下,TrkA 可能导致细胞死亡,强调神经元存活对营养因子的依赖性。有趣的是,它表现出对 NGF 的抗性,持续激活 AKT1 和 NF-kappa-B,但无法激活 Ras-MAPK 信号级联。TrkA 还拮抗抗增殖 NGF-NTRK1 信号传导,促进神经元前体分化。同种型 TrkA-III 另外在血管生成中发挥作用,并在过度表达时表现出致癌活性。

种属

Human

表达系统

HEK293

标签

C-hFc;C-His

蛋白编号

P04629 (M1-P382)

基因 ID
蛋白结构
N-term
TrkA (M1-P382)
Accession # P04629
hFc-His
C-term
同用名
High affinity nerve growth factor receptor; Trk-A; NTRK1; MTC; TRK
分子量

85-95 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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