1. 重组蛋白
  2. Receptor Proteins Enzymes & Regulators
  3. Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Trk Receptors TrkA
  5. TrkA 蛋白, Rat (HEK293, His)

TrkA 蛋白, Rat (HEK293, His)

目录号: HY-P73460
COA 产品使用指南

TrkA 蛋白是一种受体酪氨酸激酶,对于中枢和周围神经系统发育、调节神经元增殖、分化和存活至关重要。作为一种高亲和力 NGF 受体,TrkA 在配体结合后发生同二聚化和自磷酸化,激活下游效应器(SHC1、FRS2、SH2B1、SH2B2、PLCG1)级联。TrkA 蛋白, Rat (HEK293, His) 是重组的 TrkA 蛋白,由 HEK293 表达,带有 C-His 标签。TrkA 蛋白, Rat (HEK293, His) 全长 386 个氨基酸,分子量约为 ~70-95 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥460 In-stock
10 μg ¥790 In-stock
50 μg ¥2200 In-stock
100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The TrkA protein is a receptor tyrosine kinase that is critical in central and peripheral nervous system development, regulating neuronal proliferation, differentiation, and survival. As a high-affinity NGF receptor, TrkA undergoes homodimerization and autophosphorylation upon ligand binding, activating downstream effectors (SHC1, FRS2, SH2B1, SH2B2, PLCG1) cascade. TrkA Protein, Rat (HEK293, His) is the recombinant rat-derived TrkA protein, expressed by HEK293 , with C-His labeled tag. The total length of TrkA Protein, Rat (HEK293, His) is 386 a.a., with molecular weight of ~70-95 kDa.

研究背景

TrkA 蛋白是一种受体酪氨酸激酶,通过调节交感神经元和感觉神经元的增殖、分化和存活,在中枢和周围神经系统的发育和成熟中发挥着至关重要的作用。作为 NGF(其主要配体)的高亲和力受体,TrkA 也可以被 NTF3/neurotropin-3 激活,尽管 NTF3 通过 NTRK1 特异性支持轴突延伸而不影响神经元存活。在二聚体 NGF 配体结合后,TrkA 经历同二聚化、自磷酸化和激活,随后招募、磷酸化和/或激活下游效应子,例如 SHC1、FRS2、SH2B1、SH2B2 和 PLCG1。这些效应器调节不同但重叠的信号级联,指导细胞存活和分化。通过 SHC1 和 FRS2,TrkA 激活控制细胞分化和存活的 GRB2-Ras-MAPK 级联,而通过 PLCG1,它调节 NF-Kappa-B 激活和对细胞存活至关重要的基因转录。此外,通过 SHC1 和 SH2B1,TrkA 控制 Ras-PI3 激酶-AKT1 信号级联,进一步促进细胞存活的调节。在没有配体和激活的情况下,TrkA 可能促进细胞死亡,强调神经元存活对营养因子的依赖性。

生物活性

Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 13.22 ng/mL in the presence of 10 ng/mL of rrNGF, corresponding to a specific activity is 7.564×10^4 U/mg.

  • Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 13.22 ng/mL in the presence of 10 ng/mL of rrNGF,corresponding to a specific activity is 7.564×104 U/mg
种属

Rat

表达系统

HEK293

标签

C-His

蛋白编号

P35739 (A33-P418)

基因 ID
蛋白结构
N-term
TrkA (A33-P418)
Accession # P35739
His
C-term
同用名
High affinity nerve growth factor receptor; Trk-A; NTRK1; MTC; TRK
氨基酸序列

AASCRETCCPVGPSGLRCTRAGTLNTLRGLRGAGNLTELYVENQRDLQRLEFEDLQGLGELRSLTIVKSGLRFVAPDAFHFTPRLSHLNLSSNALESLSWKTVQGLSLQDLTLSGNPLHCSCALLWLQRWEQEDLCGVYTQKLQGSGSGDQFLPLGHNNSCGVPSVKIQMPNDSVEVGDDVFLQCQVEGQALQQADWILTELEGTATMKKSGDLPSLGLTLVNVTSDLNKKNVTCWAENDVGRAEVSVQVSVSFPASVHLGKAVEQHHWCIPFSVDGQPAPSLRWFFNGSVLNETSFIFTQFLESALTNETMRHGCLRLNQPTHVNNGNYTLLAANPYGQAAASIMAAFMDNPFEFNPEDPIPVSFSPVDTNSTSRDPVEKKDETP

分子量

Approximately 70-95 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
TrkA Protein, Rat (HEK293, His)
目录号:
HY-P73460
需求量: