TRMT112 蛋白, Human (His-SUMO)

TRMT112 Protein activates various methyltransferases for rRNA, tRNA, and protein modifications. Partnering with BUD23, it methylates 18S rRNA's guanine N(7). With N6AMT1/HEMK2, it catalyzes N5-methylation of ETF1 on 'Gln-185' and monomethylates 'Lys-12' of histone H4 (H4K12me1). Collaborating with ALKBH8, TRMT112 methylates 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine in specific tRNA species. With THUMPD3, it contributes to N(2)-methylguanosine formation in various tRNA substrates. TRMT112 and METTL5 methylate adenine's 6th position in 18S rRNA. Its involvement in pre-rRNA processing aids small-subunit rRNA production, and diverse interactions with BUD23, N6AMT1/HEMK2, ALKBH8, and METTL5 highlight its regulatory functions. Interactions with THUMPD3, THUMPD2, and TRMT11 underscore its intricate role in these processes. TRMT112 Protein, Human (His-SUMO) is the recombinant human-derived TRMT112 protein, expressed by E. coli, with N-His, N-SUMO labeled tag. The total length of TRMT112 Protein, Human (His-SUMO) is 125 a.a., with molecular weight of ~30.2 kDa.

TRMT112 蛋白可作为多种甲基转移酶的激活剂，参与 rRNA、tRNA 和蛋白质修饰。它与甲基转移酶 BUD23 合作，甲基化 18S rRNA 中鸟嘌呤的 N(7) 位，同时与 N6AMT1/HEMK2 合作，催化 ETF1 在“Gln-185”上的 N5 甲基化，并对 ETF1 的“Lys-12”进行单甲基化。组蛋白 H4 (H4K12me1)。此外，TRMT112 与 ALKBH8 结合，参与特定 tRNA 物种中反密码子环摆动位置处的 5-羧甲基尿苷甲基化为 5-甲基羧甲基尿苷。它与甲基转移酶 THUMPD3 配合，有助于在各种 tRNA 底物中形成 N(2)-甲基鸟苷。此外，TRMT112 与 METTL5 一起在 18S rRNA 1832 位腺嘌呤 6 位甲基化中发挥着至关重要的作用。它参与 rRNA 前体加工，有助于小亚基 rRNA 的产生，并且与 BUD23、N6AMT1/HEMK2、ALKBH8 和 METTL5 形成各种异二聚体，凸显了其在各种甲基化途径中的多种调节功能。与 THUMPD3、THUMPD2 和 TRMT11 的相互作用进一步强调了其在这些过程中的复杂作用。

Human

E. coli

N-His;N-SUMO

Q9UI30 (1M-125S)

51504  [NCBI]

MKLLTHNLLSSHVRGVGSRGFPLRLQATEVRICPVEFNPNFVARMIPKVEWSAFLEAADNLRLIQVPKGPVEGYEENEEFLRTMHHLLLEVEVIEGTLQCPESGRMFPISRGIPNMLLSEEETES

Approximately 30.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.