Troponin C/TNNC1 蛋白, Human (N-His)

Troponin C, represented by the TNNC1 gene, centrally regulates striated muscle contraction. In the troponin complex consisting of Tn-I, Tn-T, and Tn-C, Tn-I inhibits the actomyosin ATPase, while Tn-T binds to tropomyosin. Troponin C/TNNC1 Protein, Human (N-His) is the recombinant human-derived Troponin C/TNNC1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of Troponin C/TNNC1 Protein, Human (N-His) is 161 a.a., with molecular weight of approximately 19 kDa.

以 TNNC1 基因为代表的肌钙蛋白 C 是协调横纹肌收缩的中央调节蛋白。肌钙蛋白复合物由 Tn-I、Tn-T 和 Tn-C 组成，在这一调节机制中发挥着关键作用。Tn-I 充当肌动球蛋白 ATP 酶的抑制剂，而 Tn-T 提供原肌球蛋白的结合位点。特别重要的是，Tn-C 作为钙结合成分，在与钙相互作用时，它会抵消 Tn-I 对肌动蛋白丝的抑制作用。这种复杂的相互作用凸显了肌钙蛋白 C 在将钙信号转化为肌肉收缩动力学调节方面的关键作用。

Human

E. coli

N-6*His

P63316 (M1-E161)

7134  [NCBI]

MDDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGCISTKELGKVMRMLGQNPTPEELQEMIDEVDEDGSGTVDFDEFLVMMVRCMKDDSKGKSEEELSDLFRMFDKNADGYIDLDELKIMLQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMKGVE

approximately 19 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.