1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TSLP 蛋白, Cynomolgus (His)

TSLP 蛋白, Cynomolgus (His)

目录号: HY-P70703
产品使用指南

胸腺基质淋巴细胞生成素 (TSLP) 是一种造血细胞因子,通过由胸腺基质淋巴细胞生成素受体和 IL-7R α 链组成的异二聚体受体复合物发出信号。 TSLP 主要影响骨髓细胞,并促进与各种炎症性疾病(包括哮喘、过敏性炎症和慢性阻塞性肺病)的免疫相关的 2 型辅助 T (TH2) 细胞反应。TSLP 蛋白, Cynomolgus (His) 是重组的 TSLP 蛋白,由 E. coli 表达,带有 C-6*His 标签和 Q37E 突变。TSLP 蛋白, Cynomolgus (His) 全长 131 个氨基酸,分子量约为 ~16.0 kDa。

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描述

Thymic stromal lymphopoietin (TSLP) is a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. TSLP mainly impacts myeloid cells and promotes T helper type 2 (TH2) cell responses associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. TSLP Protein, Cynomolgus (His) is the recombinant cynomolgus-derived TSLP protein, expressed by E. coli , with C-6*His labeled tag and Q37E mutation. The total length of TSLP Protein, Cynomolgus (His) is 131 a.a., with molecular weight of ~16.0 kDa.

研究背景

胸腺基质淋巴细胞生成素 (TSLP) 是一种造血细胞因子,通过由胸腺基质淋巴细胞生成素受体和 IL-7R α 链组成的异二聚体受体复合物发出信号。 TSLP主要影响骨髓细胞,诱导单核细胞释放T细胞吸引趋化因子,并促进CD11c(+)树突状细胞的成熟。 TSLP 可促进与多种炎症性疾病(包括哮喘、过敏性炎症和慢性阻塞性肺病)的免疫相关的 2 型辅助 T (TH2) 细胞反应,因此 TSLP 被认为是治疗此类疾病的潜在治疗靶点。 TSLP 基因的选择性剪接产生多种亚型,较短的(主要)亚型是一种抗菌蛋白,对 B.B. 具有抗菌和抗真菌活性。蜡状芽孢杆菌、大肠杆菌、粪肠球菌、轻链球菌、表皮葡萄球菌和C.白色念珠菌[1][2][3][4]

种属

Cynomolgus

表达系统

E. coli

标签

C-6*His

蛋白编号

XP_005557555.1 (Y29-Q159, Q37E)

基因 ID

102119254  [NCBI]

蛋白结构
N-term
TSLP (Y29-Q159, Q37E)
Accession # XP_005557555.1
6*His
C-term
同用名
Thymic stromal lymphopoietin; Thymic stroma-derived lymphopoietin; TSLP
氨基酸序列

YDFTNCDFEKIEADYLRTISKDLITYMSGTKSTDFNNTVSCSNRPHCLTEIQSLTFNPTPRCASLAKEMFARKTKATLALWCPGYSETQINATQAMKKRRKRKVTTNKCLEQVSQLLGLWRRFIRTLLKKQ

分子量

Approximately 16.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TSLP Protein, Cynomolgus (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TSLP Protein, Cynomolgus (His)
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HY-P70703
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