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  3. Tubulin cofactor A 蛋白, Human

Tubulin cofactor A 蛋白, Human

目录号: HY-P73565
COA 产品使用指南

微管蛋白辅因子 A 蛋白是一种关键的微管蛋白折叠蛋白,可启动超级复合物(辅因子 A 至 E)内的微管蛋白折叠途径。辅因子 A 和 D 捕获微管蛋白并将其稳定在准天然状态。Tubulin cofactor A 蛋白, Human 是重组的 微管蛋白辅因子 A 蛋白,由 E. coli 表达,不带标签。Tubulin cofactor A 蛋白, Human 全长 108 个氨基酸,分子量约为 ~16 kDa。

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描述

The tubulin cofactor A protein is a key tubulin folding protein that initiates the tubulin folding pathway within the supercomplex (cofactors A to E). Cofactors A and D capture tubulin and stabilize it in a quasi-native state. Tubulin cofactor A Protein, Human is the recombinant human-derived Tubulin cofactor A protein, expressed by E. coli , with tag free. The total length of Tubulin cofactor A Protein, Human is 108 a.a., with molecular weight of ~16 kDa.

研究背景

微管蛋白辅因子 A 蛋白作为微管蛋白折叠蛋白发挥着至关重要的作用,积极参与微管蛋白折叠途径的初始阶段。它是由辅因子 A 至 E 组成的超级复合物的一部分。辅因子 A 和 D 通过捕获和稳定微管蛋白的准天然构象发挥着关键作用。辅因子 E 与辅因子 D-微管蛋白复合物的相互作用促进随后与辅因子 C 的结合,导致微管蛋白多肽的释放,致力于采用天然状态。在协调这些复杂的步骤时,微管蛋白辅因子 A 蛋白在微管蛋白折叠的早期阶段发挥着关键作用,有助于形成功能性且稳定的微管蛋白结构。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

O75347 (M1-A108)

基因 ID
蛋白结构
N-term
TBCA (M1-A108)
Accession # O75347
C-term
同用名
Tubulin-specific chaperone A; CFA; TBCA
分子量

Approximately 16 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of PBS, pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Tubulin cofactor A Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Tubulin cofactor A Protein, Human
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HY-P73565
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