Tyrosine Hydroxylase 蛋白, Mouse (sf9, His)

Tyrosine hydroxylase protein is a key catalyst in the biosynthesis of catecholamines, including dopamine, norepinephrine, and epinephrine, and oversees the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa) , which is the critical and rate-limiting step pathway in synthesis.Tyrosine Hydroxylase Protein, Mouse (sf9, His) is the recombinant mouse-derived Tyrosine Hydroxylase protein, expressed by sf9 insect cells , with N-His labeled tag.

酪氨酸羟化酶是一种在儿茶酚胺（包括多巴胺、去甲肾上腺素和肾上腺素）的生物合成中发挥关键作用的酶。它催化该途径中的限速步骤，通过使用四氢生物蝶呤和分子氧将 L-酪氨酸转化为 L-二羟基苯丙氨酸 (L-多巴)。虽然其主要底物是酪氨酸，但酪氨酸羟化酶也表现出较低的特异性，并且可以羟基化苯丙氨酸和色氨酸。这种酶活性对于参与各种生理过程的神经递质的合成至关重要。此外，酪氨酸羟化酶被发现在出生后发育过程中积极调节视网膜玻璃体血管的退化，表明其除了儿茶酚胺生物合成之外还具有多种作用。

Measured by its Tyrosinase activity. The specific activity is 23.938 U/mg that incubate at 37°C for 20min.

Mouse

Sf9 insect cells

N-10*His

P24529 (P2-S498)

21823

Partial

Approximately 65 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCL, 500 mM NaCl, pH 7.4, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.