PLAU/uPA 蛋白, Human (411a.a, HEK293, His)

uPA chain A Protein, a key player in the plasminogen activation system, selectively cleaves plasminogen, initiating fibrinolysis and contributing to tissue remodeling. Its precision underscores its role in regulating proteolytic activity, emphasizing its significance in the cascade of events leading to fibrinolysis and extracellular matrix remodeling in various physiological processes. PLAU/uPA Protein, Human (411a.a, HEK293, His) is the recombinant human-derived PLAU/uPA protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PLAU/uPA Protein, Human (411a.a, HEK293, His) is 411 a.a., with molecular weight of 45-55 kDa.

uPA 链 A 是纤溶酶原激活系统中的关键角色，在选择性裂解酶原纤溶酶原以生成称为纤溶酶的酶活性形式时发挥着关键作用。这种蛋白水解激活是纤维蛋白溶解的关键步骤，纤溶酶的作用是降解纤维蛋白凝块并有助于组织重塑和修复。uPA 链 A 在裂解纤溶酶原方面的精确性强调了其在调节蛋白水解活性的微妙平衡中的重要性，强调了其在导致纤维蛋白溶解和涉及细胞外基质重塑的其他生理过程的级联事件中作为关键引发剂的作用。

Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). Read at excitation and emission wavelengths of 380 nm and 460 nm (top read). The specific activity is 1120.66 pmol/min/µg, as measured under the described conditions.

Human

HEK293

C-6*His

P00749 (S21-L431)

5328  [NCBI]

SNELHQVPSNCDCLNGGTCVSNKYFSNIHWCNCPKKFGGQHCEIDKSKTCYEGNGHFYRGKASTDTMGRPCLPWNSATVLQQTYHAHRSDALQLGLGKHNYCRNPDNRRRPWCYVQVGLKLLVQECMVHDCADGKKPSSPPEELKFQCGQKTLRPRFKIIGGEFTTIENQPWFAAIYRRHRGGSVTYVCGGSLISPCWVISATHCFIDYPKKEDYIVYLGRSRLNSNTQGEMKFEVENLILHKDYSADTLAHHNDIALLKIRSKEGRCAQPSRTIQTICLPSMYNDPQFGTSCEITGFGKENSTDYLYPEQLKMTVVKLISHRECQQPHYYGSEVTTKMLCAADPQWKTDSCQGDSGGPLVCSLQGRMTLTGIVSWGRGCALKDKPGVYTRVSHFLPWIRSHTKEENGLAL

45-55 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM HEPES, 2 mM CaCl₂, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.