1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. UB2Q2 蛋白, Human (Sf9)

UB2Q2 蛋白, Human (Sf9)

目录号: HY-P701495
产品使用指南

UB2Q2 蛋白是泛素-蛋白酶体系统的一个组成部分,具有 E2 泛素结合酶的功能,擅长从 E1 复合物中接受泛素并催化其与多种蛋白质底物的共价附着。在体外,UB2Q2 有效介导“Lys-48”连接的多聚泛素化。UB2Q2 蛋白, Human (Sf9) 是重组的 UB2Q2 蛋白,由 Sf9 insect cells 表达,不带标签。UB2Q2 蛋白, Human (Sf9) 全长 374 个氨基酸。

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描述

The UB2Q2 protein is a component of the ubiquitin-proteasome system and functions as an E2 ubiquitin-conjugating enzyme, adept at accepting ubiquitin from the E1 complex and catalyzing its covalent attachment to a variety of protein substrates. In vitro, UB2Q2 efficiently mediates “Lys-48”-linked polyubiquitination. UB2Q2 Protein, Human (Sf9) is the recombinant human-derived UB2Q2 protein, expressed by Sf9 insect cells , with tag free. The total length of UB2Q2 Protein, Human (Sf9) is 374 a.a., .

研究背景

UB2Q2 是泛素-蛋白酶体系统的重要参与者,作为 E2 泛素结合酶发挥作用,能够熟练地从 E1 复合物中接受泛素并催化其与各种蛋白质底物的共价附着。在体外环境中,UB2Q2 通过有效介导“Lys-48”连接的多泛素化反应来展示其催化活性。作为泛素机制的关键贡献者,UB2Q2 在特定蛋白质的靶向降解中发挥着关键作用,从而影响由泛素依赖性蛋白水解调节的细胞过程。

种属

Human

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

Q8WVN8 (S2-G375)

基因 ID

92912

蛋白结构
N-term
UB2Q2 (S2-G375)
Accession # Q8WVN8
C-term
同用名
UBE2Q2; Ubiquitin-conjugating enzyme E2 Q2; E2 ubiquitin-conjugating enzyme Q2; Ubiquitin carrier protein Q2; Ubiquitin-protein ligase Q2
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UB2Q2 Protein, Human (Sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
UB2Q2 Protein, Human (Sf9)
目录号:
HY-P701495
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