1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. UBE2C 蛋白, Human (Sf9, His, Strep)

UBE2C 蛋白, Human (Sf9, His, Strep)

目录号: HY-P701350
产品使用指南

UBE2C 蛋白是泛素-蛋白酶体系统的关键成分,充当 E2 泛素结合酶,是泛素与靶蛋白共价连接的核心。在体外,UBE2C 熟练地促进“Lys-11”和“Lys-48”连接的多泛素化,证明了泛素链形成的多功能性。UBE2C 蛋白, Human (Sf9, His, Strep) 是重组的 UBE2C 蛋白,由 Sf9 insect cells 表达,带有 N-Strep, N-8*His 标签。UBE2C 蛋白, Human (Sf9, His, Strep) 全长 178 个氨基酸。

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描述

The UBE2C protein is a key ubiquitin-proteasome system component that acts as an E2 ubiquitin-conjugating enzyme and is the core of the covalent linkage of ubiquitin to target proteins. In vitro, UBE2C expertly promotes “Lys-11” and “Lys-48” linked polyubiquitination, demonstrating the versatility of ubiquitin chain formation. UBE2C Protein, Human (Sf9, His, Strep) is the recombinant human-derived UBE2C protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of UBE2C Protein, Human (Sf9, His, Strep) is 178 a.a., .

研究背景

UBE2C 是泛素-蛋白酶体系统的关键组成部分,作为 E2 泛素结合酶发挥作用,在泛素与靶蛋白的共价连接中发挥核心作用。在体外,UBE2C 在促进“Lys-11”和“Lys-48”连接的多泛素化方面表现出催化能力,展示了其在泛素链形成中的多功能性。至关重要的是,UBE2C 是后期促进复合物/环体 (APC/C) 中不可或缺的因子,APC/C 是一种细胞周期调节的泛素连接酶,对于控制有丝分裂进展至关重要。它在 APC/C 底物上启动“Lys-11”连接的多聚泛素链中的作用强调了其在协调蛋白酶体降解特定蛋白质方面的重要性,从而促进有丝分裂的及时和受控退出。

种属

Human

表达系统

Sf9 insect cells

标签

N-Strep;N-8*His

蛋白编号

O00762 (A2-P179)

基因 ID

11065

蛋白结构
N-term
8*His-Strep
UBE2C (A2-P179)
Accession # O00762
C-term
同用名
UBE2C; Ubiquitin-conjugating enzyme E2 C; (E3-independent) E2 ubiquitin-conjugating enzyme C; E2 ubiquitin-conjugating enzyme C; UbcH10; Ubiquitin carrier protein C; Ubiquitin-protein ligase C
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UBE2C Protein, Human (Sf9, His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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UBE2C Protein, Human (Sf9, His, Strep)
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HY-P701350
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