1. 重组蛋白
  2. Ubiquitin Related Proteins
  3. Ubiquitin Enzymes
  4. E2 Enzymes
  5. UBE2D4
  6. UBE2D4 蛋白, Human (GST)

UBE2D4 蛋白, Human (GST)

目录号: HY-P71400
产品使用指南

UBE2D4 蛋白是一种重要的泛素-蛋白酶体系统成分,充当 E2 泛素结合酶,接受 E1 复合物中的泛素并催化其与蛋白质的共价附着。在体外,UBE2D4 通过促进所有七个泛素 Lys 残基的多泛素化而表现出多功能性,可能有利于“Lys-11”和“Lys-48”连接的多泛素化。这种动态泛素链范围表明 UBE2D4 参与多种细胞过程和调节途径,突出了其在蛋白质降解和周转的复杂网络中的重要性。UBE2D4 蛋白, Human (GST) 是重组的 UBE2D4 蛋白,由 E. coli 表达,带有 N-GST 标签。UBE2D4 蛋白, Human (GST) 全长 147 个氨基酸,分子量约为 ~40.0 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

UBE2D4 Protein, a vital ubiquitin-proteasome system component, acts as an E2 ubiquitin-conjugating enzyme, accepting ubiquitin from the E1 complex and catalyzing its covalent attachment to proteins. In vitro, UBE2D4 demonstrates versatility by promoting polyubiquitination with all seven ubiquitin Lys residues, potentially favoring 'Lys-11' and 'Lys-48'-linked polyubiquitination. This dynamic ubiquitin chain range suggests UBE2D4's involvement in diverse cellular processes and regulatory pathways, highlighting its significance in the intricate network of protein degradation and turnover. UBE2D4 Protein, Human (GST) is the recombinant human-derived UBE2D4 protein, expressed by E. coli, with N-GST labeled tag. The total length of UBE2D4 Protein, Human (GST) is 147 a.a., with molecular weight of ~40.0 kDa.

研究背景

UBE2D4 是泛素-蛋白酶体系统的重要组成部分,通过接受 E1 复合物中的泛素并催化其与其他蛋白质的共价连接,充当 E2 泛素结合酶。在体外,UBE2D4 通过展示使用所有七个泛素 Lys 残基促进多聚泛素化的能力,展示了其多功能性,并可能偏好“Lys-11”和“Lys-48”连接的多聚泛素化。泛素链连接的这种动态范围表明 UBE2D4 参与多种细胞过程和调节途径,强调其在蛋白质降解和周转的复杂网络中的重要性。

种属

Human

表达系统

E. coli

标签

N-GST

蛋白编号

Q9Y2X8 (M1-M147)

基因 ID

51619  [NCBI]

蛋白结构
N-term
GST
UBE2D4 (M1-M147)
Accession # Q9Y2X8
C-term
同用名
Ubiquitin-Conjugating Enzyme E2 D4; HBUCE1; Ubiquitin Carrier Protein D4; Ubiquitin-Protein Ligase D4; UBE2D4; UBCH5D
氨基酸序列

MALKRIQKELTDLQRDPPAQCSAGPVGDDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPEIAHTYKADREKYNRLAREWTQKYAM

分子量

Approximately 40.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UBE2D4 Protein, Human (GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
UBE2D4 Protein, Human (GST)
目录号:
HY-P71400
需求量: