UBE2G2 蛋白, Human (GST)

UBE2G2 Protein, a vital ubiquitination component, accepts ubiquitin from the E1 complex, catalyzing its covalent attachment to various proteins, with a preference for 'Lys-48'-linked polyubiquitination. Its specificity in shaping ubiquitin chains underscores its role in cellular processes. Intricately associated with endoplasmic reticulum-associated degradation (ERAD), UBE2G2 maintains cellular homeostasis by regulating protein turnover. Indispensable for sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase, it facilitates subsequent proteasomal degradation. UBE2G2 Protein, Human (GST) is the recombinant human-derived UBE2G2 protein, expressed by E. coli, with N-GST labeled tag. The total length of UBE2G2 Protein, Human (GST) is 165 a.a., with molecular weight of ~43.0 kDa.

UBE2G2 是泛素化机制中的重要组成部分，通过接受 E1 复合物中的泛素并催化其与各种蛋白质的共价附着，在细胞过程中发挥着关键作用。在体外，UBE2G2 表现出对“Lys-48”连接的多聚泛素化的特定催化偏好，突出显示其参与塑造具有特定连接的泛素链。值得注意的是，UBE2G2 与内质网相关降解 (ERAD) 密切相关，强调了其通过调节蛋白质周转来维持细胞稳态的重要性。此外，UBE2G2 对于甾醇诱导的 3-羟基-3-甲基戊二酰辅酶 A 还原酶泛素化是必不可少的，从而促进其随后的蛋白酶体降解。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-GST

P60604 (M1-L165)

7327  [NCBI]

MAGTALKRLMAEYKQLTLNPPEGIVAGPMNEENFFEWEALIMGPEDTCFEFGVFPAILSFPLDYPLSPPKMRFTCEMFHPNIYPDGRVCISILHAPGDDPMGYESSAERWSPVQSVEKILLSVVSMLAEPNDESGANVDASKMWRDDREQFYKIAKQIVQKSLGL

Approximately 43.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.