UBE2M 蛋白, Human

UBE2M Protein is a crucial mediator in the ubiquitin-like protein NEDD8 conjugation pathway. It accepts NEDD8 from the UBA3-NAE1 E1 complex, covalently attaching it to target proteins like CUL1, CUL2, CUL3, and CUL4, particularly interacting with the E3 ubiquitin ligase RBX1. This specificity in neddylating specific targets suggests a pivotal role in regulating cellular processes, notably contributing to cell proliferation. UBE2M Protein, Human is the recombinant human-derived UBE2M protein, expressed by E. coli, with tag free. The total length of UBE2M Protein, Human is 183 a.a., with molecular weight of ~25.0 kDa.

UBE2M 蛋白作为泛素样蛋白 NEDD8 缀合途径中的重要介质，接受来自 UBA3-NAE1 E1 复合物的 NEDD8 并催化其与各种靶蛋白的共价附着。与 E3 泛素连接酶 RBX1（与 RBX2 不同）的独特相互作用强调了其对特定靶标（包括 CUL1、CUL2、CUL3 和 CUL4）进行 neddylation 的特异性。这种特定的 neddylation 活性表明在调节细胞过程中发挥着关键作用，特别是有助于细胞增殖。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

Tag Free

P61081 (M1-K183)

9040  [NCBI]

MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

Approximately 25.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 2 mM DTT, 150 mM NaCl, 10% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.