UCHL3 蛋白, Rat (His)

The UCHL3 protein is a deubiquitinating enzyme (DUB) that complexly controls cellular ubiquitin levels by processing ubiquitin precursors and ubiquitinated proteins. As a thiol protease, UCHL3 selectively recognizes and hydrolyzes the peptide bond of ubiquitin or the C-terminal glycine of NEDD8, showing a 10-fold preference for Arg and Lys at the P3 position, and has a special preference for "Lys-48" linked ubiquitin chains. affinity. UCHL3 Protein, Rat (His) is the recombinant rat-derived UCHL3 protein, expressed by E. coli , with N-His labeled tag. The total length of UCHL3 Protein, Rat (His) is 229 a.a., with molecular weight of ~28 kDa.

UCHL3 蛋白是一种去泛素化酶 (DUB)，通过处理泛素前体和泛素化蛋白来复杂地控制细胞泛素水平。作为硫醇蛋白酶，UCHL3 选择性识别并水解泛素或 NEDD8 C 端甘氨酸的肽键，对 P3 位的 Arg 和 Lys 表现出显着的 10 倍偏好，并对“Lys-48”连接的特殊亲和力泛素链。在顶室中，UCHL3 使 ENAC 去泛素化，从而精细调节顶膜回收。它的影响延伸到胰岛素信号传导和脂肪生成，间接增强 IGFIR、AKT 和 FOXO1 的磷酸化。UCHL3 对于视网膜、骨骼肌和生殖细胞维持的压力反应至关重要，也可能在工作记忆中发挥作用。值得注意的是，它表现出水解 UBB(+1)（一种抵抗蛋白酶体降解的泛素突变形式）的能力，强调了其在细胞过程和稳态动态控制中的重要性。

The specific activity of UCHL3 was 68.654 nmoL/min/mg in DUB assay using ubiquitin-based proluciferin as substrate.

Rat

E. coli

N-His

Q91Y78 (E2-A230)

498560  [NCBI]

EGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMEPELLSMVPRPVCAVLLLFPITEKYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGLIHAIANNKDKMHFESGSALKKFLEESVAMSPEERARHLENYDAIRVTHETSAHEGQTEAPSIDEKVDLHFIALVHVDGHLYELDGRKPFPINHGKTSDETLLEDAIEVCKKFMERDPDELRFNAIALSAA

Approximately 28 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.