ULK3 蛋白, Human (sf9, His, GST)

ULK3 Protein, Human (sf9, His, GST) is the recombinant human-derived ULK3, expressed by sf9 insect cells , with His, GST labeled tag. ,

ULK3 是一种丝氨酸/苏氨酸蛋白激酶，作为 Sonic hedgehog (SHH) 信号通路和自噬的调控因子。在缺乏 SHH 配体时，ULK3 通过与 SUFU 相互作用抑制其蛋白激酶活性，阻止 GLI 蛋白（GLI1、GLI2 和/或 GLI3）的磷酸化，从而负调控 SHH 信号通路。而在 SHH 存在时，ULK3 从 SUFU 上解离，发生自磷酸化并介导 GLI2 的磷酸化，激活 GLI2 并促进其核转位。体外实验显示，ULK3 可磷酸化 GLI2，但对 GLI1 和 GLI3 的磷酸化效率较低。此外，ULK3 在细胞衰老后能够诱导自噬，参与自噬调控。

Human

Sf9 insect cells

N-His;N-GST

Q6PHR2-1 (A2-Q472)

25989

Partial

Approximately 81.1 kDa

Greater than 80% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.