1. 重组蛋白
  2. Receptor Proteins
  3. UNC5B 蛋白, Rat (HEK293, Fc)

UNC5B 蛋白, Rat (HEK293, Fc)

目录号: HY-P76120
COA 产品使用指南

UNC5B 是 netrin-1 的依赖性受体。它对血管生成过程中的血管分支进行负面调节。此外,UNC5B 还保护髓鞘并部分缓解损伤诱导的神经病理性疼痛 (NP)。UNC5B 在没有与 netrin-1 结合的情况下参与轴突的引导和外源性凋亡信号通路的正向调节。UNC5B 蛋白, Rat (HEK293, Fc) 是重组的 UNC5B 蛋白,由 HEK293 表达,带有 C-hFc 标签。UNC5B 蛋白, Rat (HEK293, Fc) 全长 347 个氨基酸,分子量为 72-90 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥470 In-stock
10 μg ¥800 In-stock
50 μg ¥2200 In-stock
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

UNC5B is a netrin-1-dependent receptor. It negatively regulates vascular branching during angiogenesis. In addition, UNC5B protects myelin and partially alleviates injury-induced neuropathic pain (NP). UNC5B is involved in the guidance of axons and the positive regulation of exogenous apoptosis signaling pathways without binding to netrin-1. UNC5B Protein, Rat (HEK293, Fc) is the recombinant rat-derived UNC5B protein, expressed by HEK293 , with C-hFc labeled tag. The total length of UNC5B Protein, Rat (HEK293, Fc) is 347 a.a., with molecular weight of 72-90 kDa.

研究背景

UNC5B 通过 AMPKULK1 的磷酸化促进 Schwann 细胞 (SC) 中的自噬通量,这取决于其配体 netrin-1,从而保护髓鞘并部分缓解损伤诱导的神经病理性疼痛 (NP)[1]
UNC5B 在没有与 netrin-1 结合的情况下参与轴突的引导和外源性凋亡 (apoptosis) 信号通路的正向调节[2]
UNC5B 的激活抑制了芽生性血管生成,从而将 UNC5B 确定为潜在的抗血管生成靶点[3]

生物活性

Measured by its binding ability in a functional ELISA. Immobilized UNC5B at 5 µg/mL (100 µL/well) can bind rmNetrin-1. The ED50 for this effect is 218.2 ng/mL

种属

Rat

表达系统

HEK293

标签

C-hFc

蛋白编号

NP_071543.1 (G27-D373)

基因 ID

60630  [NCBI]

蛋白结构
N-term
UNC5B (G27-D373)
Accession # NP_071543.1
hFc
C-term
同用名
Netrin receptor UNC5B; p53RDL1; UNC5B; P53RDL1; UNC5H2
氨基酸序列

GIDSGGQALPDSFPSAPAEQLPHFLLEPEDAYIVKNKPVELHCRAFPATQIYFKCNGEWVSQKGHVTQESLDEATGLRIREVQIEVSRQQVEELFGLEDYWCQCVAWSSSGTTKSRRAYIRIAYLRKNFDQEPLAKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPAQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQACQKTACTTVCPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCVLNQRTLNDPKSRPLEPSGD

分子量

Approximately 90 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

UNC5B Protein, Rat (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
UNC5B Protein, Rat (HEK293, Fc)
目录号:
HY-P76120
需求量: