2. 重组蛋白
  3. USP10 蛋白, Human (Sf9, His, GST)

USP10 蛋白, Human (Sf9, His, GST)

目录号: HY-P702584
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规格 价格 是否有货 数量
10 μg ￥2600 In-stock
50 μg ￥7000 In-stock
100 μg   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

生物活性

The fundamental role of USP10 is specific removal of ubiquitin from substrates. USP10 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535nM emission light under the excitation condition of 485nM.

种属

Human
表达系统

Sf9 insect cells
标签

His;GST
蛋白编号

Q14694 (A2-L798)
基因 ID

9100
同用名
USP10; Ubiquitin carboxyl-terminal hydrolase 10; Deubiquitinating enzyme 10; Ubiquitin thioesterase 10; Ubiquitin-specific-processing protease 10
分子量

Approximately 114.7 kDa
纯度

Greater than 85% as determined by reducing SDS-PAGE.
性状

溶液
组分

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5.
内毒素含量

<1 EU/μg, determined by LAL method.
复溶方法

Please use rapid thawing with running water to thaw the protein.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (210 KB)

产品使用指南 (1213 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称：
USP10 Protein, Human (Sf9, His, GST)
目录号：
HY-P702584
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