USP21 蛋白, Human

USP21 protein is a multifunctional deubiquitinase that plays a key role in the regulation of epigenetic transcriptional repression by deubiquitinating histone H2A. As a coactivator, it relieves the inhibition of dimethylation and trimethylation of histone H3 at "Lys-4", thereby regulating transcription initiation. USP21 Protein, Human is the recombinant human-derived USP21 protein, expressed by E. coli , with tag free.

USP21 是一种多功能去泛素酶，通过特异性去泛素化组蛋白 H2A，在转录抑制的表观遗传调控中发挥关键作用，充当共激活剂并缓解组蛋白 H3 在“Lys-4”处的二甲基化和三甲基化的抑制，从而调节转录起始。这种调节功能通过组蛋白 H2A 去泛素化延伸至基因表达调节。此外，USP21 通过去泛素化稳定 BAZ2A/TIP5，证明其参与维持蛋白质稳定性。此外，USP21 具有从 NEDD8 缀合物中去除 NEDD8 的能力，而不影响 Sentrin-1 缀合物。值得注意的是，它通过介导 40S 核糖体蛋白 RPS10/eS10 和 RPS20/uS10 的去泛素化，充当核糖体质量控制 (RQC) 的负调节因子，从而抵消 ZNF598 介导的 40S 泛素化并影响细胞过程的复杂动态。

The fundamental role of USP21 is specific removal of ubiquitin from substrates. USP21 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

E. coli

Tag Free

Q9UK80 (P2-L565)

27005

Partial

Approximately 60 kDa, based on SDS-PAGE under reducing conditions.

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.