1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. USP22 蛋白, Human (Sf9)

USP22 蛋白, Human (Sf9)

目录号: HY-P701443
产品使用指南

USP22 蛋白是 SAGA 复合物中的一种组蛋白去泛素化酶,可催化组蛋白 H2A 和 H2B 去泛素化,并充当共激活剂。MYC 等激活剂有助于其招募至基因启动子,这对于转录激活至关重要。USP22 蛋白, Human (Sf9) 是重组的 USP22 蛋白,由 Sf9 insect cells 表达,不带标签。USP22 蛋白, Human (Sf9) 全长 524 个氨基酸。

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     可免费申领三个不同产品的试用装。

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描述

USP22 protein is a histone deubiquitinating enzyme in the SAGA complex that catalyzes the deubiquitination of histones H2A and H2B and acts as a coactivator. Activators such as MYC facilitate its recruitment to gene promoters, which is critical for transcriptional activation. USP22 Protein, Human (Sf9) is the recombinant human-derived USP22 protein, expressed by Sf9 insect cells , with tag free. The total length of USP22 Protein, Human (Sf9) is 524 a.a., .

研究背景

USP22 蛋白在转录调节组蛋白乙酰化 (HAT) 复合物 SAGA 中充当组蛋白去泛素化成分。该酶在催化组蛋白 H2A 和 H2B 去泛素化中发挥着至关重要的作用,在该过程中充当共激活剂。MYC 等激活剂可促进其招募至特定基因启动子,并且事实证明它对于转录激活至关重要。此外,USP22 是核受体介导的反式激活所必需的,并有助于细胞周期进程的调节,突显了其在关键细胞过程中的多方面参与。USP22 在 SAGA 复合体中的动态相互作用强调了它在协调转录事件和细胞稳态中的重要性。

种属

Human

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

Q9UPT9 (V2-E525)

基因 ID

23326

蛋白结构
N-term
USP22 (V2-E525)
Accession # Q9UPT9
C-term
同用名
USP22; Ubiquitin carboxyl-terminal hydrolase 22; Deubiquitinating enzyme 22; Ubiquitin thioesterase 22; Ubiquitin-specific-processing protease 22
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

USP22 Protein, Human (Sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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USP22 Protein, Human (Sf9)
目录号:
HY-P701443
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