Vinculin 蛋白, Human (GST)

Vinculin Protein, an actin-binding protein, is crucial for cell adhesion, morphology, and mechanosensing. It regulates E-cadherin expression and enhances mechanosensing. Vinculin forms complexes with THSD1, PTK2/FAK1, TLN1, and VCL, and interacts with APBB1IP, NRAP, CTNNB1, SYNM, SORBS1, and CTNNA1. Its interaction with CTNNA1 is necessary for localization to cell-cell junctions. Vinculin also binds to ACTN4, causing conformational changes. Vinculin Protein, Human (GST) is the recombinant human-derived Vinculin protein, expressed by E. coli, with N-GST labeled tag. The total length of Vinculin Protein, Human (GST) is 234 a.a., with molecular weight of ~53.0 kDa.

Vinculin 蛋白是一种肌动蛋白丝 (F-actin) 结合蛋白，广泛参与细胞-基质粘附和细胞-细胞粘附，在细胞形态、运动和机械传感中发挥关键作用。它调节细胞表面 E-钙粘蛋白的表达，并通过 E-钙粘蛋白复合物增强机械传感。Vinculin 表现出自缔合特性，并与 THSD1、PTK2/FAK1、TLN1 和 VCL 形成复合物。它与 APBB1IP、NRAP、TLN1、CTNNB1、SYNM、SORBS1 和 CTNNA1 相互作用，并且它与 CTNNA1 的相互作用对于其定位到细胞-细胞连接和调节 E-钙粘蛋白表达是必需的。此外，Vinculin 与 ACTN4 结合，引发构象变化。

Human

E. coli

N-GST

P18206 (2P-235E)

7414  [NCBI]

PVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVE

Approximately 53.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.