1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. VCPIP1 蛋白, Human (sf9, His)

VCPIP1 蛋白, Human (sf9, His)

目录号: HY-P701435
COA 产品使用指南 技术支持

VCPIP1 蛋白是一种多功能去泛素化酶,在多种细胞过程中发挥着至关重要的作用。它是 DNA 修复以及有丝分裂后高尔基体和内质网重新组装的重要组成部分,促进 VCP 介导的高尔基体堆积和 tER 形成。VCPIP1 蛋白, Human (sf9, His) 是重组的 VCPIP1 蛋白,由 sf9 insect cells 表达,带有 C-8*His 标签。

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规格 价格 是否有货 数量
10 μg ¥3200 In-stock
50 μg ¥9000 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The VCPIP1 protein is a multifunctional deubiquitinating enzyme that plays a crucial role in a variety of cellular processes. It is an integral component of DNA repair and postmitotic Golgi and endoplasmic reticulum reassembly, promoting VCP-mediated Golgi stacking and tER formation. VCPIP1 Protein, Human (sf9, His) is the recombinant human-derived VCPIP1 protein, expressed by sf9 insect cells , with C-8*His labeled tag.

研究背景

VCPIP1 蛋白是一种多方面的去泛素化酶,在多种细胞过程中发挥着重要作用。它复杂地参与 DNA 修复以及有丝分裂后高尔基体和内质网的重新组装,促进 VCP 介导的高尔基体堆栈重新组装和过渡内质网 (tER) 的形成。此外,VCPIP1 参与包含 STX5A、NSFL1C 和 VCP 的三元复合物的解离。此外,在 ATMATR 磷酸化后,VCPIP1 通过催化 SPRTN 去泛素化、促进 SPRTN 募集到染色质并促进共价 DNA-蛋白质交联 (DPC) 的蛋白水解裂解来促进 DNA 修复。该酶通过水解“Lys-11”和“Lys-48”连接的多聚泛素链进一步证明了其多功能性。在微生物感染的情况下,VCPIP1通过催化A型肉毒杆菌神经毒素轻链(LC)去泛素化来调节A型肉毒杆菌神经毒素(BoNT/A)中毒的持续时间,从而防止LC被蛋白酶体降解并加速肉毒杆菌神经毒素中毒在患者中。这凸显了 VCPIP1 在细胞稳态和应对外部挑战中的多样化且关键的功能。

生物活性

Ubiquitin-Rhodamine 110 (Ub-Rho110) is a fluorogenic rhodamine-based substrate. Ub-Rho110 is an ubiquitin substrate whose C-terminal derivatives are Rho110. While rhodamine is in the Ub-Rho110 moiety, it is di substituted thereby quenching the intrinsic fluorescence. However, mono-substituted rhodamine, which exhibits intense fluorescence, is released by the DUBs, thus allowing the real-time monitoring of the sensitivity or activity of DUBs by monitoring the 535 nm emission wavelengths after using 485 nm excitation. The increase in fluorescence is proportional to the DUB activity.

种属

Human

表达系统

Sf9 insect cells

标签

C-8*His

蛋白编号

Q96JH7 (M1-S1222)

基因 ID

80124

蛋白结构
N-term
VCPIP1 (M1-S1222)
Accession # Q96JH7
8*His
C-term
蛋白长度

Full Length

中文名
VCPIP1 蛋白
同用名
VCPIP1; Deubiquitinating protein VCPIP1; Valosin-containing protein p97/p47 complex-interacting protein 1; Valosin-containing protein p97/p47 complex-interacting protein p135; VCP/p47 complex-interacting 135-kDa protein
分子量

136.3 KDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

溶液

组分

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol 1 mM DTT.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

Please use rapid thawing with running water to thaw the protein.

保存条件 & 期限

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

VCPIP1 Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
VCPIP1 Protein, Human (sf9, His)
目录号:
HY-P701435
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